Histology, Biochemistry and Molecular Imaging Core

组织学、生物化学和分子成像(HBMI)核心将提供肌肉骨骼 组织病理学、组织形态计量学、生物化学、细胞和分子成像和分析服务，以支持肌肉骨骼研究中心(CMSR)的协作临床和基础科学部分，所有这些都在一个单一的管理结构下。HBMI核心内部服务的扩展和整合将进一步提高生产力，提高核心的效率，并使组织学和组织形态测量数据与潜在的生化、细胞和分子机制之间能够进行转换。此外，HBMI核心将开发新的技术和方法来生成和分析肌肉骨骼科学中的组织学、组织形态计量学、生物化学、细胞和分子数据，这将加快CMSR内所有资助项目的研究步伐。整体而言 核心的具体目标是继续：(1)提供高效和高质量的组织学， 该中心的主要目标是(1)在肌肉骨骼科学领域提供组织形态计量学、生物化学、细胞和分子成像及分析服务；(2)接触到研究助理教授(RAP)和未获资助的内科科学家(UPS)，他们也将通过利用尖端组织学、生化和分子技术在肌肉骨骼科学领域提供独特且高技能的指导来协助核心研究；(3)通过开发新技术和综合方法创新HBMI肌肉骨骼基础和临床研究。与具体目标2和3相关的主要目标是开发：1)数字全幻灯片成像(奥林巴斯公司的NanoZomer)技术，利用VisioPharm公司的图像分析软件捕获图像，用于自动定量分析骨和软骨组织形态测量的标准参数和新参数，并使用标准化的OsteoMetrics组织形态计量学系统验证这些参数；2)使用NanoZomer和VisioPamm图像分析软件或OsteoMetrics组织形态计量学系统，通过免疫组织化学(IHC)或原位杂交(ISH)分析骨折骨痂、骨和软骨组织样本中的蛋白质和基因表达；3)建立小鼠和人类组织的组织微阵列(TMA)，以优化IHC的蛋白质表达分析，并使用NanoZomer系统自动定量表达；以及4)使用BioTek Synergy Mx多模式微孔板阅读器进行基于细胞的新型高通量分析，以筛选调节肌肉骨骼细胞增殖、分化和凋亡的重要信号通路相互作用。组织学和组织形态计量学数据以及潜在的生化、细胞和分子机制之间的转换将由HBMI联席董事(马修·J·希尔顿博士、PI和Brendan Boyce博士；Co-PI)推动，他们将监督核心的所有组成部分，并协助开发新技术，以支持CMSR资助的研究。

The Histology, Biochemistry, and Molecular Imaging (HBMI) Core will provide musculoskeletal histopathology, histomorphometry, biochemistry, cellular, and molecular imaging and analysis services to support the collaborative clinical and basic science components of the Center for Musculoskeletal Research (CMSR) all under a single administrative structure. The expansion and integration of services within the HBMI Core will further increase productivity, enhance the efficiency of the Core, and enable the translation between histological and histomorophometric data and the underlying biochemical, cellular, and molecular mechanisms. Additionally, the HBMI Core will develop novel technologies and approaches to generate and analyze histology, histomorphometry, biochemistry, cellular, and molecular data in the musculoskeletal sciences, which will accelerated the pace of research for all funded projects within the CMSR. The overall Specific Aims of the Core are to continue to: (1) Provide efficient and high quality histology, histomorphometry, biochemistry, cellular, and molecular imaging and analysis services within the musculoskeletal sciences; (2) Provide access to Research Assistant Professors (RAP) and Unfunded Physician Scientists (UPS), who will also assist the Core by providing unique and highly skilled mentoring within the musculoskeletal sciences through the utilization of cutting edge histological, biochemical, and molecular technologies; and (3) Innovate HBMI musculoskeletal basic and clinical research by developing novel technologies and integrated approaches. The primary goals related to Specific Aim 2 and 3 are to develop: 1) digital whole slide imaging (Olympus' NanoZoomer) technology to capture images for automated quantitative analysis of standard and novel parameters of bone and cartilage histomorphometry using VisioPharm's image analysis software and validate these parameters using the standardized OsteoMetrics histomorphometry system; 2) quantitative methodology using the NanoZoomer and VisioPharm image analysis software or the OsteoMetrics histomorphometry system to analyze protein and gene expression by immunohistochemistry (IHC) or in situ hybridization (ISH) in tissue samples of fracture callus, bone and cartilage; 3) Tissue Microarrays (TMAs) of murine and human tissues to optimize IHC analysis of protein expression and quantify expression automatically using the NanoZoomer system; and 4) novel high throughput cell based assays using the BioTek Synergy Mx multi-mode microplate reader to screen for important signaling pathway interactions that regulate musculoskeletal cell proliferation differentiation, and apoptosis. The translation between histological and histomorophometric data and the underlying biochemical, cellular, and molecular mechanisms will be facilitated by the HBMI Co-Directors (Dr, Matthew J. Hilton; PI and Dr. Brendan Boyce; Co-PI), which will oversee all of the components of the Core and assist in the development of new technologies to support funded research in the CMSR.