FRUCTOSE LEVELS AFTER SOFT DRINK CHALLENGE IN TEENAGERS WITH OBESITY AND DIABETE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 肥胖症在美国的流行与果糖和蔗糖消费量的增加有关。许多软饮料中添加的高果糖玉米糖浆(HFCS)含有55%的果糖和45%的葡萄糖。随着软饮料消费量的增加，被归类为肥胖的人口比例也在增加。高水平的果糖摄入被证明与视网膜病变、肾病、尿酸增加、乳酸增加和胰岛素抵抗有关。 这项研究的重点是青少年以及他们摄入含有HFCS的软饮料后会发生什么。青少年将被招募并分成三组，每组10人：一组是健康对照组，一组是超重青少年(定义为BMI和GT；85%)，另一组是患有2型糖尿病的青少年。受试者将被要求彻夜禁食至少10小时。上午将测量生命体征、人体测量、皮肤皱褶厚度和生物阻抗。将获得24小时食品召回和3天食品记录。实验室将包括：果糖、葡萄糖、糖化血红蛋白、胰岛素、C肽、电解质和脂类。然后，受试者被要求喝一种已知含有HFCS的软饮料。然后，在摄入包括果糖、葡萄糖、胰岛素和瘦素的食物后，每隔15分钟抽取一次血样，持续120分钟。 了解这些水平将有助于确定健康患者与糖尿病或肥胖症患者之间的果糖代谢差异。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The obesity epidemic in the U.S. has been linked to increased consumption of fructose and sucrose. High fructose corn syrup (HFCS) added to many soft drinks contains 55% fructose and 45% glucose. As the amount of soft drink consumption has increased , so has the percentage of the population classified as obese. High levels of fructose consumption have been shown to be associated with retinopathy, nephropathy, increased uric acid, increased lactate, and insulin resistance. This study will focus on teenagers and what happens after they ingest a soft drink that contains HFCS. Teenagers will be recruited and placed into three groups of 10 each: one group will be healthy controls, one group of teens who are overweight (defined as BMI >85th percentile), and one group of teens with type 2 diabetes mellitus. The subjects will be required to fast for at least 10 hours overnight. In the morning, vital signs, anthropometrics, skin fold thickness, and bioimpedence will be measured. 24 hour food recall and 3 day food record will be obtained. Labs will be drawn including: fructose, glucose, HbA1c, insulin, C peptide, electrolytes, and lipid panel. The subjects will then be asked to drink a soft drink known to contain HFCS. Blood samples will then be drawn every 15 minutes for 120 minutes after ingestion that will include fructose, glucose, insulin, and leptin. Knowledge of these levels will help to determine how fructose metabolism differs between patients who are healthy versus patients with diabetes or obesity.