EFFECTS OF SPECIFIC SPERMATID-EXPRESSED Y CHROMOSOME GENES ON SPERM FUNCTION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 Y染色体曾经被认为只负责通过睾丸决定基因Sry来决定男性的性别。然而，随后的研究揭示了位于这条染色体上的许多其他基因，它们可能编码对雄配子的形成和功能至关重要的信息。 Y染色体曾经被认为只负责通过睾丸决定基因Sry来决定男性的性别。然而，随后的研究揭示了位于这条染色体上的许多其他基因，它们可能编码对雄配子的形成和功能至关重要的信息。 这个项目的目标是确定某些Y编码基因的作用。更具体地说，我们试图(1)揭示编码在Y染色体长臂上的基因的功能和作用机制；(2)测试睾丸决定基因Sry是否在精子功能中发挥作用；(3)确定足以产生能够成功受精的配子的最小Y基因互补。这将通过分析缺乏Y染色体不同部分的独特小鼠模型，以及使用辅助生殖技术(ART)：卵胞浆内单精子注射(ICSI)和体外受精(IVF)，结合分子生物学方法来完成。 Y染色体男性特定区域的缺失是导致生精失败的常见遗传原因，使携带受影响Y染色体的男性成为通过抗逆转录病毒疗法治疗不育的目标群体。鉴于在人类中使用抗逆转录病毒疗法存在的已知和潜在问题，有必要继续努力查明不孕的根本原因。我们的工作将促进对Y染色体编码基因在精子发生和精子功能中作用的了解，并最终可能有助于对抗美国的一个主要健康问题-不育症。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Y chromosome was once thought to be responsible only for turning on sex determination towards the male pathway via the testis determinant gene Sry. However, subsequent studies revealed a number of other genes located on this chromosome that are likely to encode information important for formation and function of the male gametes. The Y chromosome was once thought to be responsible only for turning on sex determination towards the male pathway via the testis determinant gene Sry. However, subsequent studies revealed a number of other genes located on this chromosome that are likely to encode information important for formation and function of the male gametes. The goal of this project is to define the roles of certain Y encoded genes. More specifically, we seek to (1) unveil the function and mechanism of action of genes encoded on the Y chromosome long arm, (2) test whether testis determinant gene Sry plays a role in sperm function; (3) define the minimum Y gene complement that is sufficient to generate gametes capable of achieving successful fertilization. This will be done through the analysis of unique mouse models lacking various parts of the Y chromosome, and use of assisted reproduction technologies (ART): intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF), in combination with molecular biology approaches. Deletions within the male specific region of the Y chromosome are a common genetic cause of spermatogenic failure, making men carrying affected Y chromosome a target group for infertility treatment via ART. Given the known and potential problems associated with the use of ART in humans, it is essential to continue efforts on identifying the underlying causes of infertility. Our work will advance understanding of the Y chromosome encoded genes role in spermatogenesis and sperm function and ultimately may help to combat infertility, a major health problem in U.S.A.