IMPROVED QUANTIFICATION, COVERAGE/DYNAMIC RANGE, SENSITIVITY, AND THROUGHPUT

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 核心2旨在通过多种技术进步来改善蛋白质组分析。 这些进步是通过以下方式实现的：将能力扩展到包括非常规的纳克大小和更小的样品，通过增强MS测量的动态范围，通过应用分析完整蛋白质的新方法，通过应用高pH反相分馏以改善蛋白质组覆盖，通过样品处理的进步，通过改善用氧-18的稳定同位素标记，通过实施通用的定量参考标准，通过应用iTRAQ定量，通过在应用MRM测量时增加靶向定量蛋白质组学的灵敏度，通过开发磷酸肽的多重定量策略，通过应用“下一代”蛋白质组学LC-IMS-MS仪器平台开发的帮助下，该中心的蛋白质组学系统的整体峰值容量增加的同时，样品急剧增加通过提高生物流体蛋白质组覆盖率，通过在MS灵敏度方面取得额外进展，以及在液相色谱分离方面的进一步进展，可以提高生物流体的通量。 NCRR补充，促进范式转变到人口蛋白质组学的描述。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Core 2 seeks to improve proteome analyses through a multitude of technological advancements. These advancements have been achieved by extending the capabilities to include non-conventional nanogram-sized and smaller samples, by enhancing the dynamic range of the MS measurements, by applying new methods of analyzing intact proteins, through the application of high pH reversed phase fractionation for improved proteome coverage, by advancements in sample processing, by improving stable isotope labeling with oxygen-18, by implementing a universal reference standard for quantification, through the application of iTRAQ quantification, by increasing the sensitivity of targeted quantitative proteomics when applying MRM measurements, by developing a multiplexed quantification strategy for phosphopeptides, by applying a "next generation" proteomics LC-IMS-MS instrument platform developed with the help of the Center for increasing the overall peak capacity of a proteomics system accompanied by a dramatic increase in sample throughput, by improving biofluid proteome coverage, by making additional advances in MS sensitivity, and further advancements in liquid chromatography separations. A description of NCRR supplements that facilitate the paradigm shift to population proteomics is provided.