SULFUR OXIDATION IN C ELEGANS BY OXIDATIVE STRESS AS COMPARED TO THE AGING PROC

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 C.由于秀丽线虫寿命短，交配容易控制，因此它非常适合作为衰老研究的模式生物。雅各布斯实验室在C. elegans和衰老，最近致力于氧化应激的作用。他们与其他人一起开发了OxICAT系统，用于绘制氧化还原活性巯基蛋白质组。他们的结论表明，虽然氧化应激和衰老都导致可逆氧化半胱氨酸残基的水平相当，但靶蛋白是不同的。然而，他们目前的方法只能映射氧化成二硫化物的半胱氨酸，而不能映射氧化成亚磺酸或磺酸的半胱氨酸。与Jakobs实验室合作，我们建议使用硫磺XANES来绘制总硫氧化态和老化过程导致的不可逆硫氧化量，与氧化应激相比。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. C. elegans as a model organism is well suited for studies in aging due to its short life span and easily controlled mating. The Jakobs lab has worked extensively on C. elegans and aging, most recently working on the role of oxidative stress. They, with other have developed the OxICAT system for mapping the redox active thiol proteome. Their conclusions show that while oxidative stress and aging both lead to comparable levels of reversibly oxidized cysteine residues, target proteins are different. However, their current methods can only map cysteines oxidized to disulphides and not cysteines oxidized to sulfunic or sulfonic acids. In conjunction with the Jakobs lab, we propose to use Sulfur XANES to map the total sulfur oxidation state and the amount of irreversible sulfur oxidation that happens as a result of the aging process compared to that of oxidative stress.