XAS OF THE INTERMEDIATE FORMED DURING THE REACTIONS OF P450S AND PEROXYNITRITE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 典型地，过氧亚硝酸盐（PN）通过酪氨酸硝化使硫醇盐连接的血红素蛋白失活。这些被硝化的蛋白质被检测到，并被认为在帕金森等疾病中发挥作用。老年痴呆症？S. 它们还被证明在神经退行性疾病和心血管疾病中具有显著的存在。 Ullrich和同事报道，PN与P450的反应？S得到P450化合物II（Fe（IV）氢氧化物物种，类似于细胞色素P450的反弹中间体）。 的？P450-II？如此形成的不寻常之处在于它相对稳定并且可以高产率生产。 P450-PN中间体的产率和稳定性与使用更传统的氧化剂（例如过乙酸或间氯过苯甲酸）生成P450-II时获得的结果形成鲜明对比。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Typically peroxynitrite (PN) inactivates thiolate-ligated hemes proteins through tyrosine nitration. These nitrated proteins are detected and thought to play a role in diseases such as Parkinson?s and Alzheimer?s. They also have been shown to have a significant presence in neurodegenerative and cardiovascular disorders. Ullrich and coworkers reported that the reaction of PN with P450?s yielded P450 compound II (an Fe(IV)hydroxide species, similar to the rebound intermediate of cytochrome P450). The ?P450-II? thus formed was unusual in that it was relatively stable and could be produced in high yield. The yield and stability of the P450-PN intermediate are in stark contrast to the results obtained when P450-II is generated with more traditional oxidants (e.g. peracetic acid or meta-chloroperbenzoic acid).