STRUCTURAL STUDIES OF THE ACTIN ORGANIZERS SPIRE AND FMN2

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 Spire和Cappuccino是两个肌动蛋白组织者，它们共同诱导果蝇卵母细胞极化。对其哺乳动物同源基因的研究表明，人类Spir1和Fmn2之间存在这种信号联系。为了更好地了解这些蛋白质在分子水平上是如何相互作用的，我们单独结晶了一个~25 kDa的人类Spir1区域，或者与其结合元件Fmn2的多肽形成了复合体。高分辨率的衍射数据将通过NE-CAT在APS 24ID-E获得。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Spire and Cappuccino are two actin organizers that collaborate to induce oocyte polarization in Drosophila. Studies with their mammalian orthologues have demonstrated this signaling link exists between human Spir1 and Fmn2. To better understand how these proteins interact at a molecular level, we crystallized a ~25 kDa region of human Spir1 alone, or in complex with a peptide corresponding to its binding element of Fmn2. High resolution diffraction data will be obtained at APS 24ID-E via NE-CAT.