BIOCHEMICAL ANALYSIS OF CENTROSOMAL PROTEIN COMPLEXES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 中心体是动物细胞中最主要的微管组织中心。我们的实验室有兴趣以线虫为模型，确定中心体是如何组装和发挥功能的。以前的一些实验室的工作已经确定了这种生物体中中心体功能所需的~15种蛋白质。我们目前正在使用生物化学来进一步表征这些蛋白质，并通过它们与已知中心体蛋白质的相互作用来识别更多的蛋白质。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Centrosomes are the primary microtubule organizing centers in animal cells. Our laboratory is interested in determining how centrosomes assemble and function, using C. elegans as a model. Previous work in a number of laboratories has identified ~15 proteins required for centrosome function in this organism. We are currently using biochemistry to further characterize these proteins and identify additional proteins by virtue of their interaction with known centrosomal proteins.