DEVELOPING NANOPORES AS NANOSENSORS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 纳米孔是具有纳米级半径的小孔。它们存在于自然界， 例如蛋白质α-溶血素孔[1]，但也可以人工 在硅基膜[2]和聚合物晶片[3]中制造。当浸没 在水溶液中并在施加电压的情况下， 研究带电物质的迁移，如离子或核酸。那里 纳米孔有许多有前途的应用;例如，开发DNA 测序技术或构建纳米流体装置。资源正在工作 与实验学家密切合作（Gregory Timp UIUC，Zuzanna Siwy UCI）和理论家（Jean-Pierre Leburton UIUC，Thorsten Ritz UCI）了解 合成纳米孔的物理学，并提高其作为传感器的可用性。 原子尺度的模拟是在以下方向进行的：（一）易位 通过纳米孔的DNA-蛋白质复合物;（ii）拉伸/解压缩DNA发夹 （iii）用纳米孔电容器感测DNA序列，以及 (iv)纳米孔内离子相互作用的建模。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Nanopores are small pores with nanometer-scale radii. They are found in nature, such as the proteinaceous alpha-hemolysin pore [1], but can also be artificially manufactured in silicon-based membranes [2] and polymer wafers [3]. When immersed in aqueous solution and with an applied voltage, nanopores can be used to study the translocation of charged species, such as ions or nucleic acids. There are many promising applications for nanopores; for instance, developing a DNA sequencing technique or building nanofluidic devices. The Resource is working in close collaboration with experimentalists (Gregory Timp UIUC, Zuzanna Siwy UCI) and theoreticians (Jean-Pierre Leburton UIUC, Thorsten Ritz UCI) to understand the physics of synthetic nanopores and improve their usability as sensors. Atomic-scale modeling was carried out in the following directions: (i) translocating DNA-protein complexes through nanopores; (ii) stretching/unzipping DNA hairpins with a nanopore; (iii) sensing DNA sequence with a nanopore capacitor and (iv) modeling of ion interactions inside nanopores.