CONTROL OF ULTIDRUG EFFLUX TRANSPORTER ACTIVITY BY CELL SURFACE REORGANIZATION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 本研究的两个主要目的是：一是了解多药外排转运的细胞生物学调控；二是探讨多药外排转运在胚胎发育保护和调控中的作用。这一建议连接了两个知识体系，一个是关于多药外排转运的细胞生理学，主要在癌症和上皮转运的背景下进行研究，另一个侧重于胚胎发育过程中细胞表面和膜组织的结构变化。在这个方案中，我们将研究早期胚胎发育中细胞表面的变化和外排转运蛋白活性的变化之间的关系，重点是海胆作为一种易于检测和操作的模式生物。我们还将继续努力，目前正处于该奖项的K阶段，将海胆的研究结果转化为小鼠模型，以确定这些活动变化在保护胚胎免受辅助生殖过程中潜在致畸因素的影响方面的作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The two main goals of this research are first, to understand the cell biological regulation of multidrug efflux transport and second, to probe the roles of these transporters in protection and regulation of embryonic development. This proposal links between two bodies of knowledge, one on the cellular physiology of multidrug efflux transport, primarily examined in the context of cancer and epithelial transport, and another focusing the structural changes in cell surface and membrane organization of embryo development. In this proposal, we will investigate the relationship between cell surface changes in early embryo development and changes in the efflux transporter activity focusing on sea urchins as an easily assayed and manipulated model organism. We will also continue efforts, currently ongoing in the K Phase of this award, to translate findings from the sea urchin to the mouse model, in order to characterize the role of these activity changes in protection of the embryo from potential teratogens encountered during assisted reproduction.