Initiation of Multidrug Transport at Fertilization.

描述（由申请人提供）：成功的胚胎发育需要协调执行一系列细胞事件，统称为发育程序，尽管细胞外环境不可预测。围绕受精的事件不仅启动发育程序，而且导致获得细胞防御，例如启动由多药耐药相关（mrp）转运蛋白介导的毒物/异生物质转运。受精时运输激活的机制尚不清楚，但似乎涉及转运体囊泡的移位及其插入质膜。这个建议的主要目的是，第一，描述海胆卵中的mrp转运囊泡的特征，第二，描述它们在受精后转运到质膜的过程。这个项目的结果将提供深入了解受精时胚胎表面的重组和mrp转运蛋白在发育中的作用。了解受精后mrp囊泡运输事件直接关系到更大的问题，即各种蛋白质的易位，对细胞外刺激的反应，是如何用于调节细胞和发育生理学。

DESCRIPTION (provided by applicant): Successful embryonic development requires the coordinated execution of a cascade of cellular events, collectively referred to as the developmental program, despite an unpredictable extracellular environment. The events surrounding fertilization not only initiate the developmental program, but also result in the acquisition of cellular defenses such as the initiation of toxicant/xenobiotic transport mediated by multidrug resistance-associated (mrp) transporters. The mechanism responsible for activation of transport at fertilization is unknown but appears to involve translocation of transporter vesicles and their insertion into the plasma membrane. The main aims of this proposal are, first, to characterize mrp transporter vesicles in the sea urchin egg and second, to describe their translocation to the plasma membrane following fertilization. The results of this project will provide insights into the reorganization of embryonic surfaces at fertilization and the role of mrp transporters in development. Understanding mrp vesicle trafficking events following fertilization is directly related to the larger problem of how translocation of various proteins, in response to extracellular stimuli, is employed in regulation of cellular and developmental physiology.