University of South Florida Application to Improve the SRB Mouse Barrier Facility

描述（由申请人提供）：南佛罗里达大学申请改善SRB小鼠屏障设施支持，要求提供维持可持续运行所需的设备，以支持联邦资助的需要ABSL-2遏制的研究，并在H. Lee Moffitt癌症中心和研究所的稳定研究大楼（SRB）的29,890 nsf小鼠屏障设施中隔离免疫功能低下的菌株。为了实现全新的研究能力，还需要额外的单独通风笼（IVC），包括免疫功能低下小鼠的异种移植物储存库，该储存库将作为转化药物发现的体内平台，通过现代成像模式进行监测，并与患者自身的肿瘤和治疗结果相关联，从而提高患者的个性化护理。大约70%的医学院需要小鼠服务，在过去15年中，整个项目的小鼠库存增长了2101%，仅在过去3年中，SRB设施的小鼠库存就增长了40%。所要求的设备将有助于满足这一增长轨迹，增强隔离和遏制能力，有助于建立安全的微生物屏障，在减少劳动力和总成本、用水量、洗涤剂排放和碳足迹的情况下实现可持续运营，并在有限的设施空间内将住房效率提高80%。SRB小鼠屏障设施为功能基因组服务提供空间，包括小鼠模型核心和小动物成像实验室，在严格的微生物安全设置中，毗邻坦帕湾联邦资助的研究和实验室空间的最大焦点。要求支助购买SRB设施所需的17个或13%的IVC小鼠微隔离架、9个相关空气处理机和额外的笼子装置。Tecniplast IVC单元是小鼠隔离和ABSL-2遏制的理想选择，也是可持续设计的行业领导者。该设备的净收益将增加到国家认可的生命科学研究。

DESCRIPTION (provided by applicant): University of South Florida Application to Improve the SRB Mouse Barrier Facility Support is requested for equipment critically needed to maintain a sustainable operation in support of federally-sponsored studies requiring ABSL-2 containment, and the isolation of immunocompromised strains in the 29,890 nsf mouse barrier facility of the Stabile Research Building (SRB) at the H. Lee Moffitt Cancer Center & Research Institute. Additional racks of individually ventilated caging (IVC) for mice are needed for fundamentally new research capabilities, including a repository of xenografts in immunocompromised mice that will serve as in vivo platforms for translational drug discoveries monitored with modern imaging modalities and correlated with the patient's own tumor and treatment outcomes that will improve personalized patient care. Approximately 70% of medical faculty require services for mice, inventories of which have grown 2101% program-wide in the last 15 years and 40% within the SRB facility in the last 3 years alone. The requested equipment will assist with meeting this trajectory of growth, augment isolation and containment capabilities, contribute to a secure microbiological barrier, a sustainable operation with reduced labor and total costs, water usage, detergent effluent, and carbon footprint, and increase housing efficiency by 80% in limited facility space. The SRB mouse barrier facility provides space for functional genomic services, including the Mouse Models Core and the Small Animal Imaging Laboratory in a setting of stringent microbiological security adjacent to Tampa Bay's greatest focus of federally funded research and laboratory space. Support is requested to purchase seventeen or 13% of the IVC mouse microisolator racks needed within the SRB facility, nine associated air handlers, and additional cage setups. The Tecniplast IVC units are ideal for both mouse isolation and ABSL-2 containment and the industry leader in sustainable design. The net benefit of this equipment will accrue to nationally recognized life science research.