Stem Cell and Pathology Core

干细胞和病理核心将是这个PPG的关键资源。它将为这四个项目提供广泛的试剂、程序和分析，包括i)CPC的分离、分类和表征，ii)体外CPC功能分析，以及iii)活体心脏样本的组织病理学。它将提供必要的设施和专业知识来分离、维护和操作几只基因工程小鼠的CPC。个别项目不具备一致地分离和鉴定CPC或进行广泛的组织病理学检查所需的设施或专业知识 需要进行分析。将所有方案协调会工作合并为一个核心设施将降低用品和设备的成本，因为核心将大量购买用品(从而减少开支)，而且由于维持集中库存和库存，将消除浪费和不必要的用品、试剂和设备重复。将CPC工作整合到一个核心设施中也是省时的，因为这项工作涉及的技术是非常劳动密集型的，需要专门的熟练人员。核心员工，一个由六个专职人员组成的全职团队，由以下人员直接监督 核心领导者，将提供分析的一致性和重复性。这一点至关重要，因为它将确保所有四个项目都将以相同的方式使用隔离、展开和排序的CP，从而使结果具有可比性。具有严格标准化以确保一致性的单一来源的方案控制方案将导致在所有四个项目中移植统一的方案方案人口，因此对综合和比较每个项目的成果极为重要。同样，统一的组织病理学 核心以盲目和严格标准化的方式进行分析，将有可能比较同一项目内和不同项目之间不同实验的结果。 核心用来制备CPC的方法非常有效；培养中的CPC，如 由c-kit表达决定(FACS)。第2至第10段平均为95%？3%，第10段为-95%。总而言之，核心将确保质量控制，并通过消除重复工作和最大限度地利用人员和用品，使资源得到有效利用。

The Stem Cell and Pathology Core will be a critical resource to this PPG. It will provide the four Projects with access to a wide array of reagents, procedures, and analyses, including i) CPC isolation, sorting, and characterization, ii) assays of CPC function in vitro, and iii) histopathology of cardiac samples obtained in vivo. It will provide the facilities and expertise necessary to isolate, maintain, and manipulate CPCs from several genetically engineered mice. The individual Projects do not have the facilities or expertise needed to isolate and characterize CPCs consistently or to perform the extensive histopathological analyses required. Consolidation of all CPC work into a Core facilitv will decrease the costs of supplies and eguipment because the Core will make bulk purchases of supplies (thereby reducing expenses) and because waste and unnecessary duplication of supplies, reagents, and equipment will be eliminated with the maintenance of centralized stocks and inventories. Consolidating CPC work into a Core facilitv is also time-effective because the techniques involved in this work are very labor intensive and require dedicated, skilled personnel. The Core staff, a full-time team of six dedicated individuals under the immediate supervision of the Core Leaders, will provide consistency and reproducibility of analvsis. This is crucial, because it will ensure that all four Proiects will use CPCs isolated, expanded, and sorted the same way, thereby making the results comparable. A single source of CPCs with rigorous standardization to ensure consistency will result in transplantation of uniform CPC populations in all four Proiects and thus is extremely important for the integration and comparison of results from each of the Projects. Similarly, the uniform histopathologic analyses conducted by the Core in a blinded and rigorously standardized fashion will make it possible to compare results of different experiments within the same Project and among different Projects. The methods used by the Core to prepare CPCs are extremely efficient; CPCs in culture, as determined by c-kit expression (FACS). average 95¿3% at passages 2 to 10 and remain at -95% at passage 10. In summary, the Core will ensure guality control and, by eliminating duplication of effort and maximizing the use of personnel and supplies, will enable an efficient use of resources.