Histology and Diagnostic Core

核心B的目标是为以下疾病提供组织病理学支持，包括技术和解释性支持 项目1至4。对于项目1，核心B将处理小鼠组织进行光镜分析，以 激光捕获显微解剖和电子显微镜。小鼠组织对疾病严重程度的评分将 盲目地表演。对于项目2，核心B将提供诊断专门知识，以对人类肾脏活检进行分类 HIVAN与非HIVAN肾病的光镜、免疫荧光和电子集成研究 盲目的显微镜发现。对于项目3，感兴趣的基因产品的新型试剂将是 用免疫细胞化学和免疫组织化学方法筛选正常和病变的人肾组织和小鼠组织 免疫电子显微镜。对于项目4，Nef信号调节蛋白的免疫组织化学 这些通路将在小鼠和人的肾组织上进行。

The objective of Core B is to provide histopathology support, both technical and interpretative, for Projects 1 through 4. For Project 1, Core B will process murine tissues for light microscopic analysis, for laser capture microdissection and for electron microscopy. Scoring of murine tissues for disease severity will be performed blindly. For Project 2, core B will provide diagnostic expertise to classify human renal biopsies as HIVAN vs. non-HIVAN renal disease by integration of light microscopic, immunofluorescence and electron microscopic findings in blinded fashion. For Project 3, novel reagents to gene products of interest will be screened on normal and diseased human renal tissue and murine tissues by immunocytochemistry and immunoelectronmicroscopy. For Project 4, immunohistochemistry for proteins regulated by Nef signaling pathways will be performed on murine and human renal tissues.