Mouse and cMyBP-C Protein Production Core

这一核心将设在辛辛那提儿童医院医疗中心的分子部门 心血管生物学。虽然在空间上与计划项目赠款团队的成员分开。Dr。 罗宾斯多年来一直与他们合作，并提供当前的计划项目赠款 研究人员和核心都有老鼠和蛋白质片段。 核心将是所有计划项目拨款组成部分中提出的研究的关键 负责所有转基因小鼠的设计、生产、群体维护和发货 由计划项目Grant的调查人员和核心B提出。核心也将产生不同的 体外和分离系统研究所需的蛋白质、蛋白质片段和抗体。因此，它 构成该计划项目赠款基础的一个组成部分。 核心的职责是： ？设计和构建用于生产细菌或SACU/o的MyBP-C蛋白质和蛋白质片段的所有构建物。 培养和纯化足够数量的所有蛋白质片段，以满足研究人员的需要 ？设计、制造和生成计划项目拨款中建议的所有转基因小鼠。 ？在屏障设施中保持足够数量的所有线路，以便根据需要将这些线路分发给调查人员以及要求提供这些线路的其他科学家。 ？作为cMyBP-C特异性抗体的制备、维护和质量控制的中央信息中心 ？保持上述所有物品的充分库存，并安排方便地运送给个别调查人员或核心领导。

This Core will be housed at the Cincinnati Children's Hospital Medical Center within the Division of Molecular Cardiovascular Biology. Although spatially separated from members of the Program Project Grant team. Dr. Robbins has been collaborating with them for years and supplies the current Program Project Grant investigators and Cores with both mice and protein fragments. The Core will be critical for the studies proposed in all of the Program Project Grant's Component's as it will be responsible for the design, production, colony maintenance and shipment of ail transgenic mice that are proposed by the Program Project Grant's investigators and Core B. The Core will also produce the different proteins, protein fragments and antibodies needed for the in vitro and isolated systems studies. As such, it forms an integral part of the Program Project Grant's foundation. The Core's responsibilities are: ¿ Design and build all constructs for bacterial- or Sacu/o-based MyBP-C protein and protein fragment production. ¿ Grow and purify all protein fragments in sufficient quantities to satisfy the investigators' needs ¿ Design, build and generate all transgenic mice that are proposed in the Program Project Grant. ¿ Maintain sufficient numbers of all lines in barrier facilities so that they can be distributed as needed to the investigators as well as to other scientists who request them. ¿ Serve as a central clearing house for the preparation, maintenance and quality control of the cMyBP-C specific antibodies ¿ Maintain an adequate inventory of all of the above and arrange for convenient shipping to the individual investigators or Core Leaders.