Southern Research Specialized Biocontainment Screening Center (SRSBSC)

南方研究所（南方研究所）设有南方研究专业生物防护筛选中心（SRSBSC）。参加分子图书馆计划。SRSBSC将为HTS要求生物安全等级2和3 （BSL2/3）的检测提供一个平台。目前，HTS在病毒学方面的最新进展受到现有技术的限制，这些技术主要集中在筛选基于病毒蛋白/酶的靶标或测量某些病毒的细胞病变效应。这些分析格式高度适用于HTS。然而，对文献的回顾揭示了许多实验室在分析方面的杰出创新，这些分析可以适应HTS平台，但由于这些分析中使用的仪器和报告探针的当前技术水平，这些分析不适合HTS。因此，我们将提供在网络中包括这些其他低吞吐量筛选方法的机会。

Southern Research Institute (Southern Research) offers the Southern Research Specialized Biocontainment Screening Center (SRSBSC).for participation in the Molecular Libraries Program. The SRSBSC will provide a platform for assays requiring Biosafety Levels 2 and 3 (BSL2/3) for HTS. The current state-of-the-art in HTS in virology is limited by existing technology, which is primarily focused on screening of viral protein/enzyme-based targets or measuring the cytopathic effect of some viruses. These assay formats are highly amenable to HTS. However, a review of the literature reveals outstanding innovation by numerous laboratories in assays that can be adapted to an HTS platform, but which are not amenable to HTS because of the current state-of-the art of the instrumentation and reporter probes used in these analyses. Hence we will provide the opportunity to include these other lower throughput screening methods in the Network. The Center will have five functional Cores: (1) Assay Development/Adaptation/Implementation; (2) HTS; (3) Informatics, (4) Administrative and Management and (5) Research. The overarching objective is to provide 5 assays per year that identify molecular probes for protein function and pathways involved in virus-cell interactions, which will lead to hit identification and characterization. Further, we will work with the Specialized Chemistry and/or Comprehensive Screening Centers to develop a strategic plan for hit-to-probe optimization. It is our goal to meet the following milestones in Year 1: (1) Provide oversight and logistics required to move 5 assays forward through different Cores, (2) Enhance performance of dual reporter assays to accelerate 2 screening platforms in the BSL3: Reader and microscope technologies, (3) Provide technological solutions tooptimize throughput and robustness of 5 assays for automation in BSL2/3 containment, (4) Develop 5 counter screens unique to 'the 5 screening campaigns with Assay Provider, (5) Provide screening of 5 assays of 300,000 compounds, dose response of hits and curate associated data for public dissemination. In all of these activities, we will seek to integrate our Center with the MLPCN Research Network to promote collaborations,integrative solutions and sharing of data to achieve the goals of the Molecular Libraries initiative.