Assay Development

单元1.化验方法的开发、适应和实施 单位1负责所有化验活动，以及高温超导后的鉴定，执行：(A) HTS和HCS的测定发展和适应，(B)通过确证对HITS的初步表征 化验和ECSO/ICSQ测定，(C)二次和正交化验和筛选，(D)类似物试验 和化学优化化合物，以及(E)HITS和优化的初步作用模式研究 化合物。第一单元开发和改装了36个初级HTS，从而超过了MLSCN的目标 由各种读数组成的检测，导致迄今进行了39次筛查活动。该股还 制定了27种二次/反分析方法，以支持MLSCN项目。此外，该单位还支持22次命中 使用41个剂量-反应分析的优化/合成孔径雷达项目，生成了24,000个IC50 10点曲线 重复和执行&gt；20项行动模式研究。单元1被组织成两个子单元：子单元1A将 开发和实施所有HTS测试，而子单元1B负责开发和优化软件 针对HCS的算法。

UNIT 1. ASSAY DEVELOPMENT, ADAPTATION, AND IMPLEMENTATION Unit 1 is a responsible for all assay activities, as well as for post-HTS hit characterization, performing: (a) assay development and adaptation for HTS and HCS, (b) initial characterization of hits through confirmatory assays and ECso/ICsq determination, (c) secondary and orthogonal assays and screens, (d) testing of analogs and chemistry-optimized compounds, and (e) preliminary mode-of-action studies on hits and optimized compounds. Unit 1 surpassed the MLSCN goals with the development and adaptation of 36 primary HTS assays consisting of a variety of read-outs, resulting in 39 screening campaigns thus far. The Unit also developed 27 secondary/counter assays to support the MLSCN projects. Additionally, the Unit supported 22 hit optimization/SAR projects using 41 dose-response assays, generated >24,000 IC50 10-point curves in duplicate and performed >20 mode-of-action studies. Unit 1 is organized into two subunits: subunit 1A will develop and implement all HTS assays, while subunit 1B is responsible for developing and optimizing software algorithms for HCS.