Nanopatch System for Next Generation Ion Channel Recordings

描述（由申请人提供）：该阶段II SBIR计划旨在进一步开发用于离子通道测量的电子生物科学（EBS）纳米贴片系统。所提出的系统将允许高灵敏度的测量，以前只能达到与膜片钳方法进行平面脂质双层（PLB）系统。该系统依赖于石英纳米孔膜平台上的纳米级双层和EBS设计的电子器件，以降低通常与传统PLB系统相关的噪声。该计划的目的是改善低频噪声性能以与膜片钳相媲美，调整已建立的方案并开发新方法以增加可纳入Nanopatch系统的离子通道类型，并使用具有低电导和快速动力学的细胞内通道证明系统的能力。还将生产四个原型Nanopatch系统。这种改进的Nanopatch系统将具有优于当前可用的离子通道测量装置的几个明显优点，包括低噪声、高带宽平台、稳健且可再现的双层形成、精细的温度和压力控制以及自动化双层形成和离子通道并入。所提出的Nanopatch系统将使大范围的低电导通道和具有快速动力学的通道能够在PLB中以先前无法达到的分辨率和灵敏度进行评估。

DESCRIPTION (provided by applicant): This Phase II SBIR program aims to further develop Electronic Bio Sciences' (EBS) Nanopatch system for ion channel measurements. The proposed system will allow high sensitivity measurements previously only attainable with the patch clamp method to be performed on a planar lipid bilayer (PLB) system. The system relies on a nanometer scale bilayer on the quartz nanopore membrane platform and EBS designed electronics to reduce the noise typically associated with a traditional PLB system. The aims of this program are to improve the low frequency noise performance to rival that of patch clamping, adapt established protocols and develop new methods to increase the types of ion channels that can be incorporated into the Nanopatch system and demonstrate the capabilities of the system using an intracellular channel with low conductance and fast kinetics. Four prototype Nanopatch systems will also be produced. This improved Nanopatch system will have several distinct advantages over currently available ion channel measurement apparatus including a low noise, high bandwidth platform, robust and reproducible bilayer formation, fine temperature and pressure control and automated bilayer formation and ion channel incorporation. The proposed Nanopatch system will enable a large range of low conductance channels and channels with fast kinetics to be evaluated in a PLB at previously unattainable resolution and sensitivity.