Tissue and Keratinocyte Procurement

组织和角质细胞获取核心的作用是促进初级组织的获取 以及来自正常人皮肤、患者标本和基因工程小鼠的角质形成细胞。 在基因工程小鼠中发现皮肤表型的研究人员需要确定 在人类疾病中观察到类似的表型。此外，研究人员想要确定 参与皮肤生理学的特定分子或途径需要来自基因工程的原代角质形成细胞 小鼠进行分析。皮肤生物学家经常使用人类皮肤模型，包括皮肤器官培养和 人皮肤异种移植物，其需要连续获取正常人皮肤。为了促进这些 研究，核心B将提供专业知识，服务和培训，以获得这些原代组织和 相关IRB批准。由于原代角质形成细胞培养物来源于新鲜分离的人皮肤， Core可以使用具有成本效益的统一方法分配这些组织。此外，核心B将建立 来自皮肤病理学临床实验室中储存的患者皮肤活检标本的组织库。当一个 确定皮肤或毛囊表型，相关石蜡包埋样本来自Core B组织库 将被识别并提供给核心A（皮肤组织学和表征）进行切片， 组织病理学分析。核心B还将与核心C（干细胞和异种移植物）协调， 用于异种移植的正常人类皮肤因此，核心B提供的独特服务确保了 促进皮肤的动物和人类模型中的转化研究 疾病，特别是对于没有皮肤生物学经验或接触患者标本的中心研究人员。 该中心的目标是：1）提供获得原代角质形成细胞的专业知识，服务和培训 2）提供获得福尔马林固定的正常人皮肤切片的专业知识和服务， 通过建立患者皮肤活检标本的组织库，以及3）提供 新鲜分离的正常人皮肤用于生理和病理生理研究。

The role of the Tissue and Keratinocyte Procurement Core is to facilitate the acquisition of primary tissue and keratinocytes from normal human skin, patient specimens, and genetically-engineered mice. Researchers who discover a skin phenotype in genetically-engineered mice need to identify whether a similar phenotype is observed in human disease. Additionally, researchers who want to determine if a particular molecule or pathway is involved in skin physiology require primary keratinocytes from geneticallyengineered mice for analysis. Skin biologists often use human skin models, including skin organ culture and human skin xenografts, which requires continuous procurement of normal human skin. To facilitate these studies, core B will provide expertise, service, and training in obtaining these primary tissues and the relevant IRB approvals. Since primary keratinocyte cultures are derived from freshly isolated human skin, the core can distribute these tissues using cost-effective and uniform methods. Additionally, Core B will establish a tissue bank from patient skin biopsy specimens stored in the dermatopathology clinical laboratory. When a skin or hair follicle phenotype is identified, relevant paraffin embedded samples from the Core B tissue bank will be identified and provided to Core A (Skin Histology and Characterization) for sectioning and histopathological analysis. Core B will also coordinate with Core C (Stem Cell and Xenograft) to procure normal human skin for xenografting. The unique services provided by core B therefore ensure the efficient distribution of primary tissues and facilitate translational research in animal and human models of skin disease, especially for Center researchers with no experience in skin biology or access to patient specimens. The Aims of the core are: 1) To provide expertise, service and training in obtaining primary keratinocyte cultures, 2) To provide expertise and service in obtaining formalin-fixed sections of normal human skin and human skin diseases by establishing a tissue bank of patient skin biopsy specimens, and 3) To provide freshly isolated normal human skin for physiologic and pathophysiologic studies.