NEW CHEMICAL TOOLS FOR THE QUANTITATIVE ASSESSMENT OF BIOLOGICAL METABOLOMES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 代谢酶通过激活或抑制基因表达和酶活性在调节生物功能中起关键作用。因此，系统中代谢物的定量允许生物样品的比较分析（系统生物学）。开发能够全面定量测量代谢物的技术将是恢复代谢平衡和减轻某些疾病症状的关键第一步。 为了定量和比较代谢物，必须用同位素标记物选择性地标记它们，使得分子易于通过光谱技术识别。本文提出了新的化学试剂，其将靶向最普遍的代谢物官能团，并允许对生物系统的大量关键代谢物进行定量。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Metabolites play a key role in regulating biological function by activating or inhibiting both gene expression and enzyme activity. Therefore, the quantification of metabolites in a system allows for the comparative analysis of biological samples (systems biology). The development of technologies that allow for the comprehensive quantitative measurement of metabolites will be a crucial first step in restoring metabolic balance and mitigating symptoms of certain diseases. In order to quantify and compare metabolites, they must be selectively labeled with isotopic markers such that molecules are readily recognized by spectroscopy techniques. Novel chemical reagents are herein proposed that will target the most prevalent metabolite functional groups and allow for the quantification of a large number of the key metabolites of biological systems.