PROTEOMICS CORE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 蛋白质组学核心设施的主要职责是支持INBRE研究人员和北达科他州研究社区的研究工作。 蛋白质组学核心设施将包括一个已建立的质谱实验室和一个蛋白质分离实验室。 核心设施将由在蛋白质质谱和蛋白质组学方面拥有丰富经验的John Shabb博士领导。 核心设施经理将负责设施的日常运作。 核心设施将以中等费用提供以下服务：通过二维凝胶电泳（2D-PAGE）进行蛋白质分离，二维凝胶的图像分析，以及通过质谱法对分离的蛋白质进行蛋白质鉴定和结构表征。 除了这些服务外，该设施还将为该州的本科生、研究生和博士后研究员提供教育培训课程。 培训课程将包括通过2D-PAGE进行蛋白质分离、2D-gel图像分析和通过质谱法进行蛋白质鉴定。 因此，蛋白质组学核心设施将为北达科他州的生物医学科学家和学生提供机会，将蛋白质组学的尖端技术应用到他们的研究项目中，并让学生学习当前生物医学研究中的这一重要技术。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The major responsibility of the Proteomics Core Facility is to support the research efforts of the INBRE investigators and the research community of the state of North Dakota. The Proteomics Core Facility will consist of an established mass spectrometry laboratory and a protein separation laboratory. The core facility will be directed by Dr. John Shabb who has extensive experience in protein mass spectrometry and proteomics. The core facility manager will be responsible for day-to-day operation of the facility. The core facility will offer the following services at moderate fees: protein separation by two-dimensional gel electrophoresis (2D-PAGE), image analysis of 2D-gels, and protein identification and structural characterization of the separated proteins by mass spectrometry. In addition to the services, the facility will offer educational training courses to undergraduate students, graduate students and post-doctoral fellows within the state. The training courses will include protein separation by 2D-PAGE, 2D-gel image analysis and protein identification by mass spectrometry. Thus, the Proteomics Core Facility will provide opportunities to biomedical scientists and students in North Dakota to employ the cutting edge technologies of proteomics into their research projects as well as to students to learn this important technology in the current biomedical research.