C2 - Scientific Core

项目总结(见说明)： 该计划资助项目对滥用药物的分子靶标及其相关的细胞内信号通路进行了研究，获得了与这些药物相关的细胞扰动的丰富信息。项目1-3中概述的综合研究将在现有知识的基础上扩展严格的细胞生物学、分子、生化、行为和电生理 药物治疗后纹状体神经元的研究。科学核心将致力于促进项目1-3中提议的实验。对日常工作的集中负责，如维持老鼠窝和供应通用试剂，提供了一种有效、灵活和具有成本效益的手段，以确保为所有项目提供充足的所需材料。科学核心的主要目标将是： 目的I.转基因小鼠群体的特性和维持； 目的II.关键试剂的生产，包括多克隆抗体、磷酸化状态特异性抗体、酵母双杂交筛选的设计和实施，以及AAV病毒的生产； 目的III.树突棘的分析。 所有科学核心目标都将与项目1-3的工作人员进行密切、持续的磋商，以便按要求提供服务。由于严重依赖转基因动物，科学核心公司生产的小鼠将对项目1-3中提出的许多研究至关重要。集中核心的生产将确保最佳的动物生产和使用。生化和免疫学 科学核心产生的试剂也将是项目所必需的。对树突棘的分析将是项目1的关键，也将有助于项目2和3开展其拟议的树突棘形态研究。

PROJECT SUMMARY (See instructions): Investigations into the molecular targets of drugs of abuse and their associated intracellular signaling pathways by this Program Project Grant have yielded a wealth of information regarding the cellular perturbations associated with these drugs. The combined studies outlined in Project 1-3 will extend upon this existing knowledge with rigorous cell biological, molecular, biochemical, behavioral and electrophysiological studies of striatal neurons following drug treatment. The Scientific Core will be devoted to facilitating the experiments proposed in Projects 1-3. The centralized responsibility for the performance of routine tasks, such as the maintenance of mouse colonies and the supply of common reagents, provides for an efficient, flexible and cost-effective means to ensure an adequate supply of required materials for all Projects.The major aims of the Scientific Core will be: Aim I. The characterization and maintenance of transgenic mouse colonies; Aim II. The production of key reagents, including polyclonal antibodies, phosphorylation state-specific antibodies, the design and execution of yeast two-hybrid screens, and the production of AAV viruses; Aim III. The analysis of dendritic spines. All Scientific Core Aims will be conducted in close, ongoing consultation with the staff from Projects 1-3, such that services are delivered as required. The mice produced by the Scientific Core will be essential for many of the studies proposed in Projects 1-3, due to a heavy reliance on genetically modified animals. Production by a centralized Core will ensure optimal animal production and use. The biochemical and immunological reagents generated by the Scientific Core will also be necessary for Projects. The analysis of dendritic spines will be essential for Project 1, and will also help Projects 2 and 3 to carry out their proposed studies of dendritic spine morphology.