Animal Model, Tissue/Cell Harvesting, and Phenotyping Core

核心B集中服务于它在我们目前的PPG中所扮演的角色，但是 研究犬减少，并引入小鼠成分和 表型分析作为该计划的一般服务。核心B为三个人提供所有动物模型 项目和核心C研究，并提供这些模型的非侵入性回波多普勒表型 因此，分子和细胞数据可以与完整的器官行为相关， 确认反映了其设计的主要生理功能。使用echo作为 以及终末研究压力-容积回路分析也将由该核心负责。 表1提供了核心B服务与项目和其他核心服务之间的一般互动。的 相互作用的强度由+符号的数量定性地标识。这三个项目 通过分子、细胞和亚细胞器研究， 介绍了项目1主要采用小鼠模型，但心肌细胞和心肌裂隙 将检查项目2中钙循环异常和线粒体 功能和蛋白质修饰项目3。这将使我们能够测试主 DHF和DHF之间钙循环、电生理和线粒体功能变化的结果 跨物种的CRT条件。对于犬科动物模型，核心提供主要服务生成 模型，执行必要的程序-即消融和起搏器植入，以及裂隙 收割对于小鼠模型，核心将根据需要提供整体集落维护、遗传监测和基因分型，并为其他项目提供组织和细胞。核心的第二个主要作用是为所有项目提供服务，并为每个项目提供表型分析。 动物确认其仪器和设计预期的模型确实导致 同步、不同步或非同步HF，量化影响的大小 产生的全球和区域室funcfion，并评估在这些心脏的不同步。

Core B confinues to serve the role it played in our present PPG, but with the total number of dogs to be studied reduced, and with introduction of a mouse component and incorporafion of phenotyping as a general service to the program. Core B provides all animal models to the three Projects, and Core C studies, and provides non-invasive echo-Doppler phenotyping of these models so that molecular and cellular data can be related to intact organ behavior and each model is confirmed to reflect the primary physiology it is designed to do. Mouse phenotyping using echo as well as terminal study pressure-volume loop analysis will also be the responsibility ofthis Core. Table 1 provides the general interaction of Core B services to the projects and other core. The strength of the interaction is identified qualitatively by the number of + signs. All three of the projects extensively ufilize the canine model, with both molecular, cellular and sub-organelle studies described. The mouse model is primary utilized in Project 1, but myocytes and myocardial fissue from the model will be examined for calcium cycling abnormalifies in Project 2 and for mitochondrial function and protein modifications in Project 3. This will enable us to test the consistency of primary findings in calcium cycling, electrophysiology, and mitochondrial function changes between DHF and CRT conditions across species. For the canine model, the core provides primary services generafing the model, performing necessary procedures - i.e. ablation and pacemaker implantation, and fissue harvesfing. For the mouse models, the core will provide overall colony maintenance, genetic monitoring and genotyping as required, and make tissues and cells available to the other Projects. The second major role of the Core also services all Projects, and is to provide phenotyping of each animal to confirm that the model intended by its instrumentation and design indeed resulted in synchronous, dyssynchronous, or resynchronized HF, quantify the magnitude of the effects generated on global and regional chamber funcfion, and assess dysynchrony in these hearts.