FORE-SCI TRAINING PROGRAM (RFP 08-01)

该提案的主要目的是提供脊髓损伤（SCI）研究技术的年度培训计划。该计划的参与者将在以下方面接受深入的动手实验室培训：胸椎和颈椎椎板切除术、大鼠和小鼠的脊髓挫伤和横断损伤、脊髓挫伤装置的操作、地面运动分析（BBB和BMS）、高级行为技术、脊髓损伤大鼠和小鼠的护理、脊髓组织的组织学准备和显微分析、电生理数据（包括肌电图、mep和sep）、脊髓内给药和/或细胞的方法（包括显微注射和鞘内导管给药）。该项目还将强调脊髓损伤的人性方面，包括来自SCI委员会认证的神经学家的讲座，以及与脊髓损伤患者的互动讨论，讨论他们在日常生活中面临的问题。我们还将通过讲座和讨论的形式提供相关信息，如SCI研究的历史背景、实验设计、数据分析与解释、文献追溯等。所有学员都将收到广泛的课程材料，包括插图课程手册和一些包含运动分析相关信息的dvd。该项目的目标是所有参与者在完成项目后都能熟练地进行SCI研究，并能够将在俄勒冈州立大学培训项目中学到的技术传授给他们家庭实验室的其他成员。

The major objective of this proposal is to provide an anual training program in spinal cord injury (SCI) research techniques. Participants in this program will receive in depth hands-on laboratory training in: Thoracic and cervical laminectomy surgeries, spinal contusion and transection injuries in rats and mice, operating spinal contusion injury devices, overground locomotor analysis (BBB and BMS), advanced behavioral techniques, care of SCI rats and mice, histological preparation and microscopic analysis of spinal tissue, electrophysiological readouts (including EMGs, MEPs, and SEPs), methods for intraspinal delivery of drugs and/or cells including microinjections and intrathecal catheter delivery. This program will also emphasize the human aspect of SCI by including a lecture from a SCI board certified neurologist and several interactive discussions with individuals with SCI in which the issues they face in daily life will be discussed. We also will provide relevant information via lectures and discussions on such topics as the historical background on SCI research, experimental design, data analysis and interpretation, tract tracing as well as other topics. All trainees will receive extensive course materials including an illustrated course manual and several DVDs containing locomotor analysis related information. It is the goal of the program that all participants will be proficient in performing SCI research after completion of the program and will be able to teach the techniques learned in the OSU training program to other members of their home laboratory.