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Pooled shRNA screens for target discoveries in cancer

Pooled shRNA screens for target discoveries in cancer
混合 shRNA 筛选癌症靶点发现
批准号：
8553186
负责人：
Ji Luo
金额：
$13.47万
依托单位：
DIVISION OF BASIC SCIENCES - NCI
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/8553186
关键词：
Cancer cell lineDependencyGenesGeneticGoalsHumanIntramural Research ProgramLibrariesMalignant NeoplasmsMethodsNormal CellPublishingResearch PersonnelResourcesTechnologycancer cellcost effectivesmall hairpin RNAtool

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中文摘要
翻译
背景nai已经成为一种强大的基因工具，用于剖析癌细胞的遗传依赖性。我们已经开发了方法和技术来进行快速和经济有效的shRNA文库筛选，以确定癌细胞中的遗传依赖性。在这个项目中，我们将与其他研究人员（主要是NCI内部项目的研究人员）合作，使他们能够在他们的癌细胞系中进行shRNA筛选。重要材料和方法针对大多数被注释的人类基因的shRNA文库。我们已经在不同的癌细胞系中启动了许多shRNA筛选，并且已经发表了一个这样的筛选。
英文摘要
BACKGROUNDRNAi has become a powerful genetic tool for dissecting genetic dependencies in cancer cells. We have developed methods and technologies to conduct rapid and cost-effective shRNA library screens to identify genetic dependencies in cancer cells.PURPOSEIn this project we will collaborate with other investigators (primarily in the NCI Intramural Program) to enable them to conduct shRNA screens in their cancer cell lines.SIGNIFICANT MATERIALS AND METHODSPooled shRNA library targeting most annotated human genes.FY2012 ACCOMPLISHMENTWe have initiated a number of shRNA screens in difference cancer cell lines and one such screen has been published already.
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