Cell Culture Core

核心B是中央细胞培养设施，它将为四个项目中的每一个提供支持。该核心设施将提供以下服务：1)从小鼠血液和骨髓中分离中性粒细胞（pmn）；2)小鼠肺内皮细胞的分离、表征和培养，以及3)人体肺微血管和肺动脉内皮细胞的培养，用于以下核心描述中所述项目中涉及的实验。小鼠肺内皮细胞的分离和培养是完成项目1、2、3和4中提出的研究的关键。此外。Core B还将从小鼠血液和骨髓中分离pmn，用于项目1、2和3。该中心的一项主要任务是提供来自不同敲除小鼠的肺内皮细胞和pmn；例如，TRPM2敲除、NADPH氧化酶亚基gp91（phox）敲除和pkc - α敲除小鼠的项目1；MKP5敲除、p47（phox）敲除、MK2敲除和MKP5/MK2双敲除小鼠；TRPC4淘汰赛;项目3的TRPM2敲除和Dream敲除小鼠；项目4 FoxMI转基因小鼠和iCKO小鼠。因此，这个核心的敏捷性对于项目研究的成功至关重要。

Core B is the central cell culture facility, which will provide support for each of four projects. This core facility will provide services for the following: 1) isolation of neutrophils (PMNs) from mouse blood and bone marrow; 2) isolation, characterization, and culture of mouse lung endothelial cells, and 3) culture of human lung microvascular and pulmonary artery endothelial cells for the experiments involved in the Projects as outlined in the Core description below. Mouse lung endothelial cell isolation and culture is pivotal for the accomplishment of studies proposed in Projects 1, 2, 3, and 4. In addition. Core B will also isolate PMNs from mouse blood and bone marrow for Projeds 1, 2, and 3. A major task of this core will be to provide lung endothelial cells and PMNs from different knock-out mice; e.g., TRPM2 knock-out, NADPH oxidase subunit gp91(phox) knock-out, and PKC-alpha knock-out mice for Project 1; MKP5 knock-out, p47(phox) knock-out, MK2 knock-out, and MKP5/MK2-double knock-out mice for Project 2; TRPC4 knock-out; TRPM2 knock-out, and Dream knock-out mice for Project 3; FoxMI transgenic and iCKO mice for Project 4. Thus the adivity of this Core is essential for the success of the Program Projed studies.