Cell Culture and Vector Core

细胞培养和载体核心

基本信息

项目摘要

Core B is the central cell culture and vector facility, which will provide support for each of four projects. This core facility will provide services for the following: 1) isolation of neutrophils from human and mouse blood; 2) isolation , charcterization, and culture of mouse lung endothelial cells; 3) culture of human lung micro vascular and pulmonary artery endothelial cells; 4) preparation of recombinant adenovirus expression constructs; 5) amplifying recombinant adenovirus constructs, and 6) infection of primary endothelial cells with recombinant adenoviruses for the experiments involved in the Projects as outlined in the Core description below. Mouse lung endothelial cell isolation and culture is pivotal for the accomplishment of studies proposed in Projects 1,3, and 4. In addition, these three projects will require human lung microvascular and pulmonary artery endothelial cells. The core will also isolate neutrophils from human and mouse blood for all projects. A major task of this core will be to provide viral expression constructs for the Projects 1,3, and 4. This core will also provide neutrophils and endothelial cells for the studies in Project 2. In addition, this Core is critical for providing endothelial cells from different knockout mice; e.g., NADPH oxidase (gp91 and p47 knockout), PKC-zeta knockout mice for Projects 1 and 4, TRPC4 knockout mice for Project 3, and TLR4 knockout mice for Project 4. The activity of this Core is essential for the successs of the Program Project.
核心B是中央细胞培养和载体设施,将为四个项目提供支持。该核心设施将提供以下服务:1)从人和小鼠血液中分离中性粒细胞; 2)分离、鉴定和培养小鼠肺内皮细胞; 3)培养人肺微血管和肺动脉内皮细胞; 4)制备重组腺病毒表达构建体; 5)扩增重组腺病毒。 腺病毒构建体,和6)用重组腺病毒感染原代内皮细胞,用于下面核心描述中概述的项目中涉及的实验。小鼠肺内皮细胞的分离和培养是完成项目1、3和4中提出的研究的关键。此外,这三个项目将需要人肺微血管和肺动脉内皮细胞。该核心还将为所有项目从人类和小鼠血液中分离中性粒细胞。该核心的主要任务是为项目1、3和4提供病毒表达构建体。该核心还将为项目2中的研究提供中性粒细胞和内皮细胞。此外,该核心对于提供来自不同敲除小鼠的内皮细胞是关键的;例如,NADPH氧化酶(gp 91和p47敲除),项目1和4的PKC-zeta敲除小鼠,项目3的TRPC 4敲除小鼠,以及TLR 4 4号项目的基因敲除小鼠该核心的活动对于计划项目的成功至关重要。

项目成果

期刊论文数量(0)
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会议论文数量(0)
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CHINNASWAMY TIRUPPATHI其他文献

CHINNASWAMY TIRUPPATHI的其他文献

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{{ truncateString('CHINNASWAMY TIRUPPATHI', 18)}}的其他基金

Novel E3 Ubiquitin Ligase CHFR Regulates Endothelial Barrier Integrity and Innate Immune Function
新型 E3 泛素连接酶 CHFR 调节内皮屏障完整性和先天免疫功能
  • 批准号:
    10488226
  • 财政年份:
    2021
  • 资助金额:
    $ 24.23万
  • 项目类别:
Transcription Factor Elf2 Signals Resolution of Lung Injury
转录因子 Elf2 发出肺损伤消退信号
  • 批准号:
    10363718
  • 财政年份:
    2021
  • 资助金额:
    $ 24.23万
  • 项目类别:
Novel E3 Ubiquitin Ligase CHFR Regulates Endothelial Barrier Integrity and Innate Immune Function
新型 E3 泛素连接酶 CHFR 调节内皮屏障完整性和先天免疫功能
  • 批准号:
    10297258
  • 财政年份:
    2021
  • 资助金额:
    $ 24.23万
  • 项目类别:
Transcription Factor Elf2 Signals Resolution of Lung Injury
转录因子 Elf2 发出肺损伤消退信号
  • 批准号:
    10178835
  • 财政年份:
    2021
  • 资助金额:
    $ 24.23万
  • 项目类别:
Transcription Factor Elf2 Signals Resolution of Lung Injury
转录因子 Elf2 发出肺损伤消退信号
  • 批准号:
    10586059
  • 财政年份:
    2021
  • 资助金额:
    $ 24.23万
  • 项目类别:
Endothelial TAK1 Signaling and Resolution of Pulmonary Edema in Sepsis
脓毒症肺水肿的内皮 TAK1 信号转导和解决
  • 批准号:
    9535680
  • 财政年份:
    2016
  • 资助金额:
    $ 24.23万
  • 项目类别:
Endothelial Cell Deubiquitinase A20 Signals Repair of Lung Vascular Injury
内皮细胞去泛素酶 A20 发出肺血管损伤修复信号
  • 批准号:
    9301023
  • 财政年份:
    2015
  • 资助金额:
    $ 24.23万
  • 项目类别:
TRPM2 mediates neutrophil transendothelial migration and inflammation
TRPM2介导中性粒细胞跨内皮迁移和炎症
  • 批准号:
    9260918
  • 财政年份:
    2015
  • 资助金额:
    $ 24.23万
  • 项目类别:
Endothelial Cell Deubiquitinase A20 Signals Repair of Lung Vascular Injury
内皮细胞去泛素酶 A20 发出肺血管损伤修复信号
  • 批准号:
    9105412
  • 财政年份:
    2015
  • 资助金额:
    $ 24.23万
  • 项目类别:
Ca2+ Signaling, ICAM-1 Expression, and Lung Vascular Injury
Ca2 信号传导、ICAM-1 表达和肺血管损伤
  • 批准号:
    7457949
  • 财政年份:
    2007
  • 资助金额:
    $ 24.23万
  • 项目类别:

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