Animal Models Core

动物模型核心(AMC)将通过提供 必要的转基因小鼠和体内低传代人胃肠癌的产生 小鼠体内的外植体。AMC的任务之一将是维持小鼠进行长期研究，以评估 易患癌症的人。AMC的第二项任务将是维持转基因食品的库存 菌株(例如，ELF、eIF/Smad4、Smad4、Smad3、CDK4-R24C突变体、CDK4零突变体、hTERT基因敲除)和TO 将这些菌株混合在一起，产生双突变和三突变纯合子基因的胎儿。劳作 有了组织核心B，这些胎儿将被用作胚胎成纤维细胞和肿瘤细胞的来源 这些项目。具有所需的双重或三重突变基因的幼崽将构成很大一部分 后代。为支持科研项目持续提供大量育种， 再加上基因分析以确定合适的个体将是必要的。的第三个使命 AMC将在临床医生和测试抗癌药物疗法的项目之间提供协同作用。这 协同的形式将是生产和饲养具有遗传背景的小鼠 易于制作肿瘤或裸鼠移植人肿瘤组织用于低传代繁殖 肿瘤。所有老鼠都将在屏障条件下生产和饲养，以确保持续 在AMC下，这些小鼠的无特定病原体状态、集中生产和护理将 限制接触动物的个人数量，从而进一步保障它们的健康。此外，通过 集中生产和分配这些具有遗传价值的动物，动物的核心将是 能够协调不同项目之间的使用，避免浪费，并实现规模经济 将允许以比单独进行的费用更少的费用完成这些研究。的最后一项任务 AMC将成为所有项目和核心的协同枢纽。

The Animal Models Core (AMC) will support all of the research projects in the program by providing the necessary genetically modified mice and producing low-passage in vivo human gastro-intestinal cancer explants in mice. One mission of the AMC will be to maintain mice for long-term studies to evaluate predisposition to cancer. A second mission of the AMC will be maintaining stocks of the genetically modified strains (e.g., elf, eif/Smad4, Smad4, Smad3, Cdk4-R24C mutant, cdk4 null mutant, htert knockout), and to breed these strains together to produce fetuses of double and triple mutant homozygous genotypes. Working with the Tissue Core B, these fetuses will be used as a source of embryonic fibroblasts and tumor cells by the projects. Pups with the desired double or triple mutant genotypes will constitute a large fraction of the progeny. To provide a continuous supply in support of the research projects a large amount of breeding, coupled with genotype analysis to identify the appropriate individuals will be required. A third mission of the AMC will be to provide synergy between the clinicians and projects testing anti-cancer drug therapies. This synergy will be in the form of producing, and providing husbandry of mice harboring genetic backgrounds predisposed to making tumors or nude mice xenografted with human tumor tissues for propagating lowpassage tumors. All mice will be produced and housed under barrier conditions that ensure continuing specific pathogen free status, Centralization of the production and care of these mice under the AMC will limit the number of individuals handling the animals, thereby further safeguarding their health. Moreover, by centralizing the production and dispensation of these genetically valuable animals, the animal core will be able to coordinate usage among the different projects, avoid wastage, and achieve an economy of scale that will permit these studies to be completed at less expense than if undertaken separately. A final mission of the AMC will be to serve as a synergistic hub for all projects and cores.