A spinning disk confocal microscope for live-cell imaging

描述（由申请人提供）：我们正在申请资金购买PerkinElmer UltraVIEW旋转圆盘共聚焦显微镜，用于培养细胞，组织和胚胎的实时成像。显微镜将被安置在俄亥俄州州立大学西校区分子神经生物学中心的成像核心设施中。该中心成立于25年前，是一个充满活力和互动的13名教师谁在各个学院联合任命组。该中心拥有自己的主任和支持人员以及出色的空间和资源，包括相邻的小鼠设施和斑马鱼核心设施。与此奖项一起购买的旋转圆盘共聚焦显微镜代表了关键和使能技术，将支持7个中心教师（4个主要用户和3个次要用户）的研究，他们正在研究神经科学和癌症问题，并且共同持有9个活跃的NIH赠款，总额超过240万美元。成像核心设施是由Anthony Brown博士（本提案的PI）在6年前建立的，资金来自NIH P30机构中心核心拨款。该设施由Leica TCS SL单点扫描共聚焦显微镜和带有相机和软件的Zeiss Axiophot落射荧光显微镜组成。Brown博士是该设施的主任，并监督该设施的经理Paula Monsma女士。Monsma女士负责日常运营，包括培训、用户支持、日程安排和设备维护。Brown博士是分子神经生物学中心的教授，也是活细胞成像专家，在他的研究中使用光学和电子显微镜有超过25年的经验。Brown博士保持着一个活跃而富有成效的研究计划，涉及轴突运输和神经细胞细胞骨架的活细胞成像，并在过去15年中一直得到NIH的资助。Monsma女士拥有细胞生物学硕士学位和15年的实验室研究助理经验，其中包括5年运行DNA测序核心设施和6年运行分子神经生物学中心的成像核心设施。P30中心核心补助金最近又延长了5年，为Monsma女士提供45%的工资支持，使她能够每周投入2-3天的成像核心设施职责。总而言之，这项提案利用了我们的P30机构中心核心资助所建立的专业知识和资源，以获得一项关键的新技术，该技术将支持NIH资助的9个神经科学和癌症研究项目的活细胞共聚焦成像需求。 公共卫生相关性：现代生物医学研究现在严重依赖于对活细胞、组织和生物体中荧光分子的直接观察。这笔赠款将用于购买先进的国家的最先进的共聚焦荧光显微镜用于这一目的。该共享仪器将成为俄亥俄州州立大学分子神经生物学中心成像核心设施的一部分，并将提供重要的新技术能力，以支持9个NIH资助的神经科学和癌症研究项目的研究。

DESCRIPTION (provided by applicant): We are requesting funds to purchase a PerkinElmer UltraVIEW spinning disk confocal microscope for live imaging of cultured cells, tissues and embryos. The microscope will be housed in the Imaging Core Facility of the Center for Molecular Neurobiology on the West Campus of Ohio State University. Established about 25 years ago, the Center is a dynamic and interactive group of 13 faculty who have joint appointments in various Colleges. The Center has its own Director and support staff and excellent space and resources, including an adjacent mouse facility and a zebrafish core facility. The spinning disk confocal microscope purchased with this award represents critical and enabling technology that will support the research of 7 Center faculty (4 major users and 3 minor users) who are studying problems in neuroscience and cancer, and who collectively hold 9 active NIH grants totaling more than $2.4 million dollars in annual NIH funding. The Imaging Core Facility was established 6 years ago by Dr. Anthony Brown (the PI of this proposal) with funds from an NIH P30 Institutional Center Core Grant. The facility consists of a Leica TCS SL single-point scanning confocal microscope and a Zeiss Axiophot epifluorescence microscope with camera and software. Dr. Brown is the Director of the facility and supervises the Manager of the facility, Ms. Paula Monsma. Ms. Monsma manages al day-to-day operations, including training, user support, scheduling and equipment maintenance. Dr. Brown is a Professor in the Center for Molecular Neurobiology and an expert in live-cell imaging with more than 25 years of experience using light and electron microscopy in his research. Dr. Brown maintains an active and productive research program involving live-cell imaging of axonal transport and the cytoskeleton of nerve cells and has been funded by the NIH continuously for the past 15 years. Ms. Monsma has a Masters degree in cell biology and 15 years experience as a laboratory research assistant, including 5 years running a DNA sequencing core facility and 6 years running the Imaging Core Facility in the Center for Molecular Neurobiology. The P30 Center Core grant, which was recently renewed for another 5 years, provides 45% salary support for Ms. Monsma, enabling her to devote 2-3 days a week to her Imaging Core facility duties. In summary, this proposal leverages the expertise and resources established by our P30 Institutional Center Core Grant to acquire a critical and enabling new technology that will support the live-cell confocal imaging needs of 9 NIH-funded research projects in neuroscience and cancer. PUBLIC HEALTH RELEVANCE: Modern biomedical research is now critically dependent on direct observations of fluorescent molecules in living cells, tissues and organisms. This grant wil fund the purchase of an advanced state-of-the-art confocal fluorescence microscope for this purpose. The shared instrument will form part of the Imaging Core Facility in the Center for Molecular Neurobiology at Ohio State University, and it will provide vital new technological capabilities to support the research of 9 NIH-funded research projects in neuroscience and cancer.