Gene Function Manipulation Core

操纵基因表达的能力是分子研究必不可少的基本工具。模型 删除或过表达基因的生物体、方法和工具提供了研究和 描述复杂生物现象的分子基础。基因组生物学最新进展 而RNA干扰在哺乳动物细胞中起作用的发现，现在为类似的研究提供了基础。 哺乳动物细胞的研究。事实上，几乎每一个基于分子生物学的项目现在都需要使用 RNAi和表达克隆。在过去的几年里，P01的研究人员开发了 全面的工具来操纵哺乳动物细胞中的基因表达。此外，我们还开发了一个 该机构拥有进行高通量RNAi和表达筛选的设备和专业知识。 基于这些进展，我们已经创建了一个基因功能操作核心，以支持本领域的项目。 P01。具体来说，该核心将提供慢病毒递送的短发夹RNA（shRNA）试剂和cDNA 克隆人变成了项目调查员此外，该机构将与P01研究人员合作， 和过表达筛选以阐明被病毒癌蛋白干扰的途径的组分。 虽然这种试剂可以在基因的基础上开发，但这一核心设施将提供 本发明提供了以综合方式操纵基因表达的试剂和手段， 成本效益。因此，进入和使用这一设施将加快每个项目的进展， 包括这个节目。

The ability to manipulate gene expression is a fundamental tool essential for molecular studies. In model organisms, methods and tools to delete or overexpress genes have provided the means to study and characterize the molecular basis of complex biological phenomenon. Recent advances in genome biology and the discovery that RNA interference operates in mammalian cells now provide a foundation for similar studies in mammalian cells. Indeed, nearly every molecular biologically based project now requires the use of RNAi and expression clones. Over the past several years, investigators in this P01 have developed comprehensive tools to manipulate gene expression in mammalian cells. In addition, we have developed a facility that has the equipment and expertise to perform high throughput RNAi and expression screens. Based on these advances, we have created a gene function manipulation core to support the projects in this P01. Specifically, this core will provide lentivirally delivered short hairpin RNA (shRNA) reagents and cDNA clones to program investigators. In addition, this facility will work with P01 investigators to perform shRNA and overexpression screens to elucidate components of the pathways perturbed by viral oncoproteins. Although such reagents can be developed on a gene-by-gene basis, this core facility will provide the reagents and means to manipulate gene expression in a comprehensive manner that is both efficient and cost effective. The access and use of this facility will thus accelerate progress for each of the projects that comprise this program.