Bone Core

该计划的骨核项目将提供加工和服务的共享设施和服务 对该计划所有项目中使用的动物模型的组织进行解释。该计划的总体目标 核心设施是为项目中的研究人员提供集中的组织学和图像分析支持。 将提供以下服务：关于终点分析的实验设计/咨询 组织学标本，用于组织学分析的骨组织加工(脱钙和不脱钙 切片，骨切片的骨组织形态计量学分析(即解释)，以及 软组织转移或其他软组织病变及其病理诊断。除了组织学之外 支持，核心将提供Faxitron显微放射照相和外围QCT服务。骨核 将继续提供专业知识，包括软组织样本的处理和脱钙硬质 石蜡中的组织标本除了使用塑料处理未脱钙的骨样外 嵌入技术。将为调查员提供静态和动态方面的培训和协助 骨组织形态计量学分析。这些服务的核心支持将提高标本的效率 通过相似的模型系统及其共同的方式分析和促进项目之间的交互 分析。该计划作为一个整体的一个重大好处将是一种标准化的分析格式 将使用不同实验方法/靶标的常见动物模型的样本 (例如CCL2、SDF-1和CXCR4、WNTS、DKK-1和PTHrP)。这将提供有价值的信息，可以 在计划中的所有项目之间进行共享和比较

The Bone Core of the Program Project will provide shared facilities and services for processing and interpretation of tissues from the animal models utilized in all projects of the program. The overall goal of the core facility is to provide centralized histologic and image analysis support for investigators in the project. The following services will be provided: experimental design/consultation regarding endpoint analyses for histologic specimens, processing of osseous tissues for histologic analysis (decalcified and undecalcified sections, bone histomorphometric analysis of osseous sections (i.e. interpretation), and histologic analysis of soft tissue metastases or other soft tissue lesions with their pathologic diagnosis. In addition to histologic support, the core will provide services of FAXITRON microradiography and peripheral QCT. The bone core will continue providing expertise that includes the processing of soft tissue specimens and decalcified hard tissue specimens in paraffin in addition to processing undecalcified osseous specimens using plastic embedding techniques. Investigators will be provided with training and assistance in static and dynamic bone histomorphometric analysis. Core support of these services will promote efficiency of specimen analysis and facilitate interactions between projects through the similar model systems and their common analyses. A significant benefit to the program as a group will be a standardized format for analysis of specimens from the common animal models that will be using different experimental approaches/targets (e.g. CCL2, SDF-1 & CXCR4, wnts, Dkk-1, and PTHrP). This will provide valuable information that can be shared and compared across the projects in the program