Bone Core

描述(申请人提供)：计划项目骨核将提供共享 该计划所有项目中使用的动物模型的组织处理和解释的设施和服务。核心设施的总体目标是为项目中的研究人员提供集中的组织学和图像分析支持。将提供以下服务：组织标本终点分析的实验设计/咨询、用于组织分析的骨组织处理(脱钙和非脱钙切片)、骨切片的骨组织形态计量学分析(即解释)以及软组织转移或其他软组织病变的组织学分析及其病理诊断。除了组织学支持外，该中心还将提供Faxitron显微放射照相和双X射线吸收测量术(DEXA)等服务。骨芯将建立在牙科学院现有的专业知识基础上，包括软组织样本和脱钙硬组织样本的处理。这些现有的活动将通过骨核的工作人员为项目中的研究人员提供便利。此外，新的加工服务 未脱钙的骨样将使用塑料包埋技术启动。核心设施将通过董事会认证兽医病理学家Thomas J.Rosol博士的专业知识提供病理损伤(硬组织和软组织)的解释。完整的静态和动态骨组织形态计量学分析将由经验丰富的骨组织形态计量师(姚)与我们的顾问和备受推崇的骨组织形态计量师Robert Recker博士共同完成。这些服务的核心支持将提高标本分析的效率，并通过类似的模型系统及其共同分析促进项目之间的互动。作为一个整体，该计划的一个显著好处是将使用不同的实验方法/靶标(例如，PAR1、SDF和CXCR4、BMPs和PTHrP)来分析来自常见动物模型的样本的标准化格式。这将提供有价值的信息，可以在计划中的各个项目之间进行共享和比较。

DESCRIPTION (provided by applicant): The Bone Core of the Program Project will provide shared facilities and services for processing and interpretation of tissues from the animal models utilized in all projects of the program. The overall goal of the core facility is to provide centralized histologic and image analysis support for investigators in the project. The following services will be provided: experimental design/consultation regarding endpoint analyses for histologic specimens, processing of osseous tissues for histologic analysis (decalcified and undecalcified sections, bone histomorphometric analysis of osseous sections (i.e., interpretation), and histologic analysis of soft tissue metastases or other soft tissue lesions with their pathologic diagnosis. In addition to histologic support, the core will provide services of FAXITRON microradiography and dual x-ray absorptometry (DEXA). The bone core will build on existing expertise in the School of Dentistry that includes the processing of soft tissue specimens and decalcified hard tissue specimens. These existing activities will be facilitated for investigators in the program through the staff of the bone core. In addition, new services of processing undecalcified osseous specimens will be initiated using plastic embedding techniques. The core facility will provide interpretation of the pathologic lesions (hard and soft tissue) through the expertise of a Board Certified Veterinary Pathologist, Dr. Thomas J. Rosol. Complete static and dynamic bone histomorphometric analysis will be performed by an experienced bone histomorphometrist (Yao) in conjunction with our consultant and highly regarded bone histomorphometrist, Dr. Robert Recker. Core support of these services will promote efficiency of specimen analysis and facilitate interactions between projects through the similar model systems and their common analyses. A significant benefit to the program as a group will be a standardized format for analysis of specimens from the common animal models that will be using different experimental approaches/targets (e.g., PAR1, SDF and CXCR4, BMPs and PTHrP). This will provide valuable information that can be shared and compared across the projects in the program.