STEM CELL PRODUCTION CORE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 间充质干细胞或骨髓基质细胞（MSC）是来自骨髓的成体干细胞的一个子集。这些细胞具有医学和治疗意义，因为它们已被证明可分化为成骨细胞、脂肪细胞、软骨细胞和肌细胞。此外，这些细胞类型已被证明可以抑制与疾病过程相关的炎症反应。由于其生物学特性，这些细胞有可能用于治疗大量遗传疾病。邦内尔博士已成功确定了从骨髓或脂肪组织中分离的恒河猴MSC的扩增和表征要求。 干细胞生产核心设施（SCPC）专注于非人灵长类动物MSC的生成，维护和分布。MSC通常从恒河猴骨髓和脂肪组织样品中分离。我们目前有一个从100多只不同年龄的恒河猴中产生的MSC细胞系库，准备分发。SCPC目前对TNPRC内的其他部门、杜兰健康科学中心和彭宁顿生物医学研究中心内的部门和中心以及全国其他研究实验室有很大的影响。 该核心生产恒河猴干细胞制剂，用于合作研究项目。为此，我们计划使用在上一个资助期开发的技术来分离干细胞（MSC和ESC），以扩大从不同年龄（胎儿，新生儿和成年人）和动物性别的动物中分离的各种干细胞类型的库。这些细胞将用于产生大型细胞库，供合作科学家在内部或外部使用。我们已经建立了一个广泛的干细胞库，供科学界用于各种年龄的动物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Mesenchymal stem cells or marrow stromal cells (MSCs) are a subset of adult stem cells from bone marrow. These cells are of medical and therapeutic interest because they have been shown to differentiate into osteoblasts, adipocytes, chondrocytes and myocytes. In addition these cell types have been shown to squelch inflammatory response associated with disease processes. Due to their biologic properties, these cells have the potential to be useful for the treatment of a large number of genetic diseases. Dr. Bunnell has successfully defined the requirements for the expansion and characterization of rhesus macaque MSCs isolated from either the bone marrow or adipose tissue. The Stem Cell Production Core Facility (SCPC) focuses on generation, maintenance and distribution of nonhuman primate MSCs. MSCs are regularly isolated from rhesus macaque bone marrow and adipose tissue samples. We presently have a bank of MSC cell lines generated from over 100 rhesus macaques of varying age prepared and ready for distribution. The SCPC presently has a large impact on other divisions within the TNPRC, and Departments and Centers within the Tulane Health Sciences Center and the Pennington Biomedical Research Center and other research labs nationally. This core produces rhesus macaque stem cell preparations for collaborative research projects. For this we plan to use the techniques developed over the last funding period to isolate stem cells (MSCs and ESCs) to expand the bank of various stem cell types isolated from animals of various ages (fetal, newborn and adults) and sexes of animals. The cells will be used to generate large cell banks for use internally or externally by collaborating scientists. We have generated an extensive bank of stem cells for use by the scientific community for various ages of animals.