GENERAL SCIENTIFIC

通用科学核心B的目的是协助动物外科、电生理学、 该计划的免疫细胞化学、透射电子显微镜和图像分析要求 项目教员。核心还协助调查人员进行各种生化分析和病毒载体 制作。核心设施包括执行拟议研究所需的人员和设备 在项目1至3中。该核心将进行一般动物准备，包括麻醉、插管、 导尿术、体温监测和生理变量的调节。核心将协助 研究人员正在为动物的创伤性脑损伤做准备，如个别项目中所述。在……里面 此外，还可以使用专业技术来修改未来可能需要的实验动物制剂 调查。核心人员将对大脑进行光和电子的灌流、固定和移除 显微镜检查。核心人员将进行免疫细胞化学程序，以及开发 更多抗体可视化的新程序。核心将处理组织以制造塑料 包埋光镜和电子显微镜分析。将通过以下方式生产高质量的薄片 该核心内的人员，并对其进行超微结构分析。已建立的图像分析程序 使用无偏见的体视学程序来量化免疫反应细胞的频率，以及 确定将利用的挫伤面积和体积。

The purpose of the General Scientific Core B is to assist investigators in animal surgery, electrophysiology, immunocytochemistry, transmission electron microscopy, and image analysis requirements for the Program Project faculty. The Core also assists investigators in various biochemical analyses and viral vector production. The Core facilities consist of personnel and equipment needed to perform the proposed studies in Projects 1 through 3. This Core will conduct general animal preparation, including anesthesia, intubation, catheterization, temperature monitoring, and regulation of physiological variables. The Core will assist investigators in preparing the animals for traumatic brain injury, as described in the individual projects. In addition, expertise is available to modify experimental animal preparations that may be required for future investigations. Core personnel will perform perfusion, fixation, and removal of brains for light and electron microscopic examination. Core personnel will carry out immunocytochemical procedures, as well as develop new procedures for the visualization of additional antibodies. The Core will process tissue for plastic embedding for both light and electron microscopic analysis. High quality thin sections will be produced by personnel within this Core and stained for ultrastructural analysis. Established image analysis procedures using non-biased stereological procedures to quantify the frequency of immunoreactive cells, as well as to determine contusion areas and volumes will be utilized.