Biochemistry and Signaling of Receptor for Advanced Glycation End Products

通过晚期糖基化终末产物受体(RAGE)的细胞信号转导导致促炎反应。RAGE介导的炎症与糖尿病、动脉粥样硬化、阿尔茨海默病等炎症性疾病密切相关。在生物化学部分，我们已经确定了介导RAGE寡聚和调节细胞表面RAGE表达的关键结构元件。我们还通过蛋白质组学完成了RAGE细胞表面复合体的初步研究。与能源部洛斯阿拉莫斯国家实验室的科学家合作，我们还开发了可广泛用于研究其他哺乳动物膜蛋白齐聚的分离式GFP标签技术。这项技术将进一步发展，以研究RAGE信号。我们还与澳大利亚新南威尔士大学的科学家合作，研究细胞表面RAGE硫醇-二硫键交换的分子机制。

Cellular signaling via receptor for advanced glycation end products (RAGE) results in pro-inflammatory responses. RAGE-mediated inflammation has been implicated in inflammatory diseases including diabetes, atherosclerosis, Alzheimers disease. In the biochemistry part, we have identified critical structural elements that mediate RAGE oligomerization and regulate RAGE expression on the cell surface. We have also completed a preliminary study of RAGE cell surface complex via proteomics. In collaboration with scientists in the Department of Energy's Los Alamos National Lab, we have also developed split GFP tagging technology that can be broadly used to study oligomerization of other mammalian membrane proteins. The technology will be further developed to study RAGE signaling. We also collaborate with scientists in University of New South Wales, Australia, to study the molecular mechanism of RAGE thiol-disulfide exchanges on the cell surface.