Transplant Models

正如本项目的目标和目的所指出的，该项目旨在创建新的模型，以检验不同的假设，并为通过造血干细胞移植治疗淋巴瘤、骨髓瘤、白血病、乳腺癌和肾癌的新策略的开发提供新的理解。这些疾病的造血干细胞移植治疗通常会导致淋巴细胞减少和免疫功能受损。在人类中，有能力在癌症治疗相关的淋巴细胞减少症的背景下上调胸腺功能的观察已经被转化为小鼠模型。我们已经开始了四项平行的努力，以确定胸腺功能的控制点。在利用KGF的实验中，我们观察到，虽然胸腺细胞前体池的大小和参与迁移的分子没有受到影响，但胸腺上皮细胞水平的关键控制点受到影响，从而增加了胸腺细胞的增殖和总数，从而增加了胸腺细胞成熟和整体胸腺生成的利基。

As noted in the goals and objectives stated for this project, it aims to create new models that test distinct hypotheses and provide new understandings for development of novel strategies in the treatment of lymphoma, myeloma, leukemia, breast cancer and renal cancer by hematopoietic stem cell transplantation. Hematopoietic stem cell transplant treatment of these diseases generally results in lymphopenia and immune compromise. The observation that in humans there is the ability to upregulate thymus function in the setting of cancer therapy-associated lymphopenia has been translated to murine models. We have initiated four parallel efforts to identify points of control in thymus function. In experiments utilizing KGF, which was found to upregulate thymus activity, we observed that while thymocyte precursor pool size and molecules involved in migration are not affected, the critical control point at the level of thymus epithelial cells are affected such that this cell population increases proliferation and total number thereby increasing niches for thymocyte maturation and overall thymopoiesis.