Characterization of the Osteosarcoma Genome

这个项目是最近启动的。进展主要是在发展必要的基础设施以实现项目目标方面。由于骨肉瘤是一种罕见的癌症，获得合适的生物标本是研究这种疾病的主要障碍。为了克服这个问题，有必要接触到内部社区之外。已与儿童肿瘤组（COG）建立了获取标本的合作，COG是儿科癌症的主要国家合作临床试验组，对COG试验下收集的标本进行测序。由于骨肉瘤已被TARGET项目（国家癌症研究所产生有效治疗的治疗适用研究，http：//target.cancer.gov/about/）选中，TARGET基础设施已被用于促进COG合作。这一合作将提供用于分析的样本。该项目早期阶段的另一个方面是开发必要的测序基础设施。为支持这一项目，安装了适当的测序和计算设备，还开发了用于数据分析的生物信息学基础设施。一组骨肉瘤细胞系的数据生成已经开始。这对于该项目的长期目标非常有用，因为良好表征的细胞系可用于本研究过程中获得的生物学重要结果的功能研究。我们现在已经开始分析通过TARGET计划收集的临床生物标本。

This project was recently initiated. Progress is primarily at the level of developing the necessary infrastructure to accomplish the project goals. As osteosarcoma is a rare cancer, obtaining suitable biospecimens is a major obstacle to studying this disease. To overcome this problem it has been necessary to reach outside of the intramural community. A collaboration to obtain specimens has been established with the Childrens Oncology Group (COG), the primary national cooperative clinical trials group for pediatric cancer, to sequence specimens collected under COG trial. As osteosarcoma had been selected by the TARGET project (NCIs Therapeutically Applicable Research to Generate Effective Treatments, http://target.cancer.gov/about/), the TARGET infrastructure has been used to facilitate the COG collaboration. This collaboration will provide samples for analysis. An additional aspect of the early phase of this project has been the development of the necessary sequencing infrastructure. Suitable sequencing and computational equipment has been installed to support this project, and the bioinformatics infrastructure for data analysis has also been developed. Data generation has begun on a panel of osteosarcoma cell lines. This will be highly useful for the long term goals of the project since well characterized cell lines can be used for functional investigation of the biologically important results obtained in the course of this research. We have now initiated the analysis of clinical biospecimens collected through the TARGET program.