Cellular Electrophysiology of Nicotine Addiction Clinical Phenotypes

描述（由申请人提供）：吸烟仍然是美国可预防疾病和死亡的主要原因，并且已知是可遗传的。本应用程序旨在开发一套基于临床定义的影像学表型和基因型的尼古丁成瘾细胞系。我们的目标是测试严重尼古丁成瘾和携带吸烟相关风险等位基因的患者对尼古丁激动剂的神经元电生理反应是否与基于临床影像学的电路表型强度相关。我们在这里的创新是紧密的

DESCRIPTION (provided by applicant): Smoking remains the leading cause of preventable diseases and death in the US, and is known to be heritable. This application aims to develop a set of nicotine addiction cell lines based on clinically defined imaging phenotype and genotype. Our goal is to test that neuronal electrophysiological response to nicotinic agonists will be associated with the strength of the clinical imaging based circuit phenotype in patients with severe nicotine addiction and carry smoking related risk alleles. Our innovation here is to closely link these cell lines with state-of-the art clinical measures of nicotine addiction, so that we coud iteratively test electrophysiological phenotypes of the cells that are likely underlying key clinicl nicotine addiction measures. PUBLIC HEALTH RELEVANCE: Smoking remains the leading cause of preventable diseases and death in the US. This application aims to develop nicotine addiction cell lines based on clinically defined imaging phenotype and genotype. Our goal is to identify electrophysiological mechanisms underlying clinical nicotine addiction.