SC COBRE: PROTEIN SCIENCE CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 创建蛋白质生物科学核心是为了协助Cobre脂质组学研究人员研究与调节脂质代谢和信号有关的蛋白质的结构和功能。多年来，核心已经从严格的咨询角色转变为在蛋白质科学的各个方面为研究人员提供服务的更积极的角色。这主要包括从大肠杆菌培养物中表达和纯化重组蛋白的通用服务。此外，还向研究人员提供了动态光散射和圆二色光谱仪等仪器，以及深入的辅导，以表征蛋白质的物理性质。最近，Core开始提供更专业的服务，包括建立稳定的哺乳动物细胞系，表达感兴趣的蛋白质，抗体设计和纯化，免疫沉淀，蛋白质晶体筛选，肽合成，蛋白质修饰，基于高效液相的分析，和脂肪蛋白质印迹。由于蛋白质生物科学核心提供的各种服务和强大的技术支持基础，它们让不熟悉蛋白质科学但对研究蛋白质结构和功能感兴趣的研究人员可以更轻松地进行实验。该中心的目标是：1)为个别研究人员生产结构和功能表征所需的感兴趣的蛋白质；2)提供各种与蛋白质相关的专业服务，以满足研究人员的需要；3)通过指导和咨询，提高研究人员在蛋白质科学方面的理解和能力。总而言之，这些目标应该有助于科布雷目标调查人员的科学成就。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Protein Bioscience Core was created to assist COBRE lipidomics investigators in studying structures and functions of proteins involved in the regulation of lipid metabolism and signaling. Over the years, the Core has transitioned itself from a strictly consultation role to a more active role in servicing investigators in various aspects of protein science. This mainly included the generic service of expressing and purifying recombinant proteins from E. coli cultures. In addition, instruments such as dynamic light scattering and circular dichroism spectrometers were made available to researchers, together with in-depth tutoring, for the characterization of the physical properties of proteins. Recently, the Core started to offer more specialized services, including generation of stable mammalian cell lines expressing protein of interest, antibody design and purification, immunoprecipitation, protein crystal screening, peptide synthesis, protein modification, HPLC-based assays, and fat Western blotting. Because of the variety of services provided by the Protein Bioscience Core and the strong technical supporting base, they allow researchers who are not familiar with protein science, but are interested in studying protein structures and functions, to perform their experiments with greater ease. The aims of the Core are: 1) To produce proteins of interest for individual investigators that are needed for structural and functional characterization; 2) To offer a variety of protein-related specialty services to suit the needs of investigators; and 3) to enhance the understanding and capability of investigators in protein science through mentoring and consultation. Taken together, these aims should contribute to the scientific achievement of the targeted COBRE investigators.