PUI RESEARCH-TOUGALOO-MAO

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在萌芽酵母中，大规模的基因缺失分析表明，在~6200个酵母基因中，超过80%是非必需的，这意味着真核细胞中的许多基因和途径必须是功能冗余的或在遗传扰动后的表型后果中得到缓冲。我对探索这种功能冗余很感兴趣，特别是当它与转录调控网络有关时。许多未知的开放阅读框架(ORF)与已知的转录因子家族具有序列同源性。使用高通量遗传分析(合成遗传阵列分析，或SGA)、DNA微阵列和全基因组定位分析或芯片上的分析，我想将这些未知的假定调控基因中的一个或多个与新的或现有的生理意义上的途径联系起来。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In the budding yeast Saccharomyces cerevisiae, large-scale gene deletion analysis has shown that over 80% of the ~6200 yeast genes are non-essential, implying that many genes and pathways in eukaryotic cells must be functionally redundant or buffered from phenotypic consequences following genetic perturbation. I am interested in exploring this functional redundancy, especially as it relates to transcriptional regulatory networks. A number of uncharacterized open reading frames (ORFs) have sequence homology to well known families of transcription factors. Using high-throughput genetic analysis (Synthetic Genetic Array Analysis, or SGA), DNA microarrays, and Genome-wide location analysis or Chip on chip, I would like to connect one or more of these uncharacterized putative regulatory genes with a new or existing physiologically significant pathway.