ECOLOGICAL GENOMICS: NICHE CHARACTERIZATION OF BOECHERA STRICTA

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 需要在自然环境环境中进行更多的研究，才能真正了解背后的机制 以确定群体的表达模式，并确定这是否源于特定适应性特征的遗传。这个 实验室实验和实地研究的结果不一致说明了跨学科的必要性 在自然环境中进行研究。 我们建立了一个近交系和母本家庭的共同花园，就在一个 评估已知应激反应途径和候选特征(防御性)的作用的自然补丁 在确定生态位边界和估计它们的进化潜力方面，生物多样性和生物多样性(包括化合物和水利用效率)。胡麻B. Stricta是拟南芥的另一个野生近亲，使基因组工具如全基因组芯片的使用成为可能。 成绩单分析(见下文方法部分)。因此，我们正在结合数量遗传学的工具， 生理生态学、化学生态学和基因组学在这一领域的实验中了解的问题是生态位 对进化生态学家来说至关重要。2010年夏天，西门子博士接待了一位来自美国旧金山大学的研究员。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The need for more studies in natural environmental settings is required to truly understand the mechanisms behind expression patterns of a population and to determine if this is due to the inheritance of specific adaptive traits. The inconsistency of results between laboratory experiments and field studies illustrates the need for interdisciplinary studies in natural environments. We established a common garden of inbred lines and maternal families of Boechera stricta within and just outside a naturally occurring patch to evaluate the role of known stress response pathways and candidate traits (defensive compounds and water use efficiency) in determining niche boundaries and to estimate their evolutionary potential. B. stricta is another close wild relative of Arabidopsis, enabling the use of genomic tools such as microarrays for genomewide transcript profiling (see below under Methods). Thus we are combining the tools of quantitative genetics, physiological ecology, chemical ecology and genomics in this field experiment to understand issues of the niche that are fundamentally important to evolutionary ecologists. Dr. Siemens hosted a fellow from USF during the summer of 2010.