VIRULENCE GENE REGULATION IN STAPHYLOCOCCUS AND ID OF SMALL MOLECULES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 金黄色葡萄球菌是主要的动物病原体之一，仍然是一个主要的公共卫生问题；由于缺乏有效的疫苗，以及在社区相关或医院相关的多种抗生素耐药菌株的出现。葡萄球菌的发病是一个复杂的多因素过程，涉及多个调控系统。调控系统的功能或基本生物学过程的转录调控是基于DNA-蛋白质和蛋白质-蛋白质对上游启动子区域的相互作用；因此，了解参与基因调控的网络的作用将有助于开发新的药物来控制和预防葡萄球菌感染。其中一个调控系统是葡萄球菌中的SarA蛋白家族。在所有已测序的金黄色葡萄球菌和表皮葡萄球菌基因组中，几乎都发现了10个SARA类似基因(SarA、SarR-V、SarX和Z、MGRA和Rot)。这些蛋白质调控许多基因，涉及调控、毒力、自溶、生物膜形成和代谢过程。调控这一基因家族及其靶基因的确切机制是我们实验室的研究重点。此外，我们还致力于开发金属氧化物纳米颗粒，以开发潜在的制剂，以控制和防止细菌在社区和医院环境中传播。2010年夏天，明迪·诺瓦克在ARRA的资助下与曼纳博士合作。她得到了4000美元，实验室得到了1000美元。曼纳博士招待了一位来自蒙大拿山的研究员。马蒂学院在2010年夏天。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Staphylococcus aureus is one of the major animal pathogens that continues to be a main public health concern; due to the lack of an effective vaccine and the emergence of multiple antibiotic-resistant strains either in community associated or in hospital associated. Staphylococcal pathogenesis is a complex multifactorial process, which involves multiple regulatory systems. The function of the regulatory systems or the transcriptional regulation of the basic biological process is based on DNA-protein and protein-protein interactions to the upstream promoter region; therefore, understanding the role of the networks involved in gene regulation will be useful for the development of new agents to control and prevent staphylococcal infections. One of these regulatory systems is the SarA protein family in Staphylococcus. A total of ten SarA paralogs (SarA, SarR-V, SarX and Z, MgrA and Rot) are found almost in all sequenced S. aureus and S. epidermidis genomes. These proteins regulate many genes involved in regulation, virulence, autolysis, biofilm formation and metabolic processes. The precise mechanisms, by which this family of genes are regulated and regulate its target genes, are the research focuses in our laboratory. Apart from these, we are also working on metal oxide nanoparticles to develop potential agents, which will control and prevent the bacterial spreading in the community and hospital settings. Mindy Novak worked with Dr. Manna with ARRA funding during the summer of 2010. She received $4000 and the laboratory received $1000. Dr. Manna hosted a fellow from Mt. Marty College during the summer of 2010.