INVESTIGATION OF DYNAMICS OF HEADPIECE PROTEINS USING NMR RELAXATION EXPERIMENTS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 蛋白质动力学研究对于理解蛋白质的各种生物学功能具有重要意义。这个项目包括一系列的自相关和互相关实验，以获得几个小模型蛋白质的详细动力学信息。具体地说，我们将研究Headpice家族的N-末端结构域和C-末端结构域的片段：人Dematin和鸡绒毛蛋白。实验将比较15N T1、T2和异核NOE，以及13C T1、13C横向C‘-C’CA、C‘-C’N和多量子化学交换C‘-N。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Studies of protein dynamics are important for understanding various biological functions of proteins. This project involves a series of auto and cross-correlation experiments to obtain a detailed dynamics information of several small model proteins. Specifically, we will look at the fragments of N and C-terminal domains of headpice family: human dematin and chicken villin. Experiments will comprize 15N t1, t2, and heteronuclear NOE, as well as 13C t1, 13C transverse C'-C'CA, C'-C'N, and multiple quantum chemical exchange C'-N.