VESOSOME

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 Vesosome是一种由载药脂质体包裹在另一个双层膜中的新型多室结构，与单层脂质体相比，Vesosome具有更好的保留和稳定性，是一种很有前途的药物载体。目前的囊泡体制备利用了饱和脂质的叉指相，这导致脂质双层在低温下形成平坦的开放片层，其在较高温度下接近形成大的单层囊泡。在这种闭合过程中，相互交叉的片层包裹其他脂质囊泡或胶体颗粒，成为囊泡体的外膜。已经表明，通过将三嵌段共聚物添加到由二棕榈醇-磷脂酰胆碱制成的叉指状片中，囊泡体达到了临床使用的适当尺寸。同时，在可见光和近红外（NIR）范围内具有吸收波长的中空金纳米球是触发药物载体内容物释放的良好候选物。已通过冷冻电子透射显微镜验证了在其水性内部隔室中含有中空金纳米球的囊泡。此外，6-羧基荧光素已被封装在一起的金球，以研究从飞秒激光脉冲照射的囊泡释放。大量的羧基荧光素被释放在响应只有几个激光脉冲。我们的研究结果表明，含有中空金纳米球的囊泡，具有大的负载能力，是有用的激光诱导的治疗剂和激光在生物系统中的其他应用的交付。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Vesosome, a new multi-compartment structure consisting of drug-loaded liposomes encapsulated within another bilayer, is a promising drug carrier with better retention and stability compared with unilamellar liposomes. The current vesosome preparation takes advantage of the interdigitated phase of saturated lipids, which causes lipid bilayers to form flat, open sheets at low temperature, that close to form large unilamellar vesicles at higher temperatures. During this closure, the interdigitated sheets encapsulate other lipid vesicles or colloidal particles to become the outer membrane of the vesosome. It has been shown that by adding the triblock copolymer to the interdigitated sheets made from dipalmitol-phosphatidylcholine, the vesosomes achieve the proper size for clinical use. Meanwhile, hollow gold nanospheres with absorbance wavelength in the visible and near infrared (NIR) range are good candidates to trigger the release of contents from drug carriers. Vesosomes containing hollow gold nanospheres in their aqueous interior compartments have been verified by cryo electron transmission microscopy. Furthermore, 6-carboxyfluorescein has been encapsulated together with gold spheres to investigate the release from vesosomes irradiated with femetosecond laser pulses. Substantial amounts of carboxyfluorescein were released in response to only a few laser pulses. Our results suggest vesosomes containing hollow gold nanospheres, with large loading capacity, are useful for laser induced delivery of therapeutic agents and other applications of lasers in biological systems.