CRYSTALLOGRAPHIC STUDIES OF MOLECULAR CHAPERONES AND RIBOZYMES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们正在继续研究分子伴侣蛋白的结构和机制，广义上说，分子伴侣蛋白有助于细胞中蛋白质和RNA分子的正确折叠、组装和靶向。 目前正在研究的系统包括“原核蛋白酶体”-一种850 kDa的复合物，它是真核蛋白酶体的简单同系物;一个被称为通用应激蛋白的“应激耐受”因子家族;一种促进细菌中膜蛋白折叠和组装的伴侣蛋白;以及一个调节细胞中RNA结构的RNA伴侣蛋白/解旋酶家族。 此外，我们正在继续研究小催化RNA或核酶的结构和机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We are continuing our studies on the structures and mechanisms of molecular chaperone proteins which, broadly speaking, facilitate the correct folding, assembly and targeting of proteins and RNA molecules in cells. Systems that are currently under study include a "prokaryotic proteasome"--a 850 kDa complex that is a simple homolog of the eukaryotic proteasome; a family of "stress endurance" factors called universal stress proteins; a chaperone that facilitates folding and assembly of membrane proteins in bacteria; and a family of RNA chaperones/helicases that modulate RNA structure in cells. Additionally, we are continuing our studies on the structure and mechanism of small catalytic RNAs, or ribozymes.