DAVID MCKAY PRT TIME

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。我们正在继续研究分子伴侣蛋白的结构和机制，广泛地说，这有助于蛋白质和RNA分子在细胞中正确的折叠、组装和靶向。目前正在研究的系统包括：一个“原核蛋白酶体”，一个850 kDa的复合体，它是真核蛋白酶体的简单同系物；一个被称为通用应激蛋白的“耐受胁迫”因子家族；一个促进细菌中膜蛋白折叠和组装的伴侣蛋白；以及一个调节细胞中RNA结构的RNA伴侣/解旋酶家族。此外，我们正在继续我们对小催化RNA或核酶的结构和机制的研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are continuing our studies on the structures and mechanisms of molecular chaperone proteins which, broadly speaking, facilitate the correct folding, assembly and targeting of proteins and RNA molecules in cells. Systems that are currently under study include a "prokaryotic proteasome", a 850 kDa complex that is a simple homolog of the eukaryotic proteasome; a family of "stress endurance" factors called universal stress proteins; a chaperone that facilitates folding and assembly of membrane proteins in bacteria; and a family of RNA chaperones/helicases that modulate RNA structure in cells. Additionally, we are continuing our studies on the structure and mechanism of small catalytic RNAs, or ribozymes.