MEDICAL STRUCTURAL GENOMICS OF PATHOGENIC PROTOZOA

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 这项建议包括确定来自真核病原体布鲁氏锥虫(非洲昏睡病)的具有医学意义的目标蛋白的初始结构。克鲁兹毛滴虫(恰加斯病)、利什曼原虫(Leishmania spp.(利什曼病)和疟原虫。(马来病)，以及小分子鸡尾酒的后续筛选，以确定结合在活性部位的化学碎片。初始结构的确定将主要取决于SEMET MAD/SAD阶段。鸡尾酒浸泡的筛选可以在固定波长下进行，但机器人自动安装和筛选的使用带来了极大的好处。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This proposal encompasses the initial structure determination of medically relevant target proteins from the eukaryotic pathogens Trypanosoma bruci (African sleeping sickness). T. cruzi (Chagas disease), Leishmania spp. (leishmaniasis) and Plasmodium spp. (maleria), and follow-up screening of small molecule cocktails to identify chemical fragments that bind at the active site. Initial structure determinations will rely primarily on SeMet MAD/SAD phasing. Screening of cocktail soaks can be done at fixed wavelength, but benifits greatly from the use of robotic automounting and screening.