CHARACTERIZATION OF ANTIGENIC PEPTIDES PRESENTED BY I-AG7

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 该项目的目标是从NOD小鼠身上鉴定由糖尿病致病的MHC II类分子I-Ag7呈递的多肽。研究将同时使用NOD小鼠和来自胰岛素瘤的细胞系。这项研究将扩展到负载、翻译后修饰和外来多肽和蛋白质的挑战的影响。将使用质谱学对这些多肽进行排序和分类，并将继续开发提高灵敏度和分析的技术。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of this project is to identify peptides presented by the diabetogenic MHC class II molecule I-Ag7 from NOD mice. Studies will use both NOD mice and cell lines derived from insulinomas. The study will extend to cover the effect of loading, posttranslational modification, and challenge by foreign peptides and proteins. Mass spectrometry will be used to sequence and catalog these peptides and the development of techniques to improve sensitivity and analysis will be pursued.