LAUE DIFFRACTION FROM MICROCRYSTALS WITH A NARROW-BANDPASS BEAM

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 先前在D1的实验已经证明了在低温和室温下使用宽带通光束从尺寸小至约30微米的蛋白质晶体收集劳厄数据的可行性。我们要求在G1的时间：（1）比较宽（25%）和窄（1.5%）带通的数据质量，（2）看看是否可以从小于10微米大小的晶体中获得有用的数据，使用G1的强摇摆器光束，（3）试图从含锌的蛋白质中获得异常数据，用于SAD定相。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Previous experiments at D1 have demonstrated the feasibility of collecting Laue data from protein crystals down to about 30 microns in size, at both cryo and room temperature, using a wide-bandpass beam. We request time at G1 to: (1) compare data quality with wide (25%) and narrow (1.5%) bandpasses, (2) see whether useful data can be obtained from crystals of < 10 microns size using the intense wiggler beam at G1, (3) attempt to obtain anomalous data from proteins containing zinc, for SAD phasing.