ID OF FACTOR CODE FOR EXPRESSION DOMAINS OF EVOLUTIONARILY FOREBRAIN ENHANCERS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 端脑在功能上分为许多不同的区域。这些区域的特点是基因表达、细胞结构和连接模式的不同组合。该领域的一个主要问题是如何指定这些区域。最近的工作已经确定了进化上保守的增强子元件，这些增强子元件调控E11.5小鼠皮质脑室区明显划分的区域的基因表达。这些区域的空间范围受多个转录因子的调控。我们的实验室对利用质谱学鉴定这些转录因子很感兴趣。我们的方法包括将特定前脑区域的核裂解物应用于增强元件。与增强子特异结合的蛋白质将被分离出来，并使用质谱学进行鉴定。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The telencephalon is divided into many functionally distinct areas. These areas are characterized by different combinations of gene expression, cytoarchitecture, and pattern of connections. A major question in the field is how these areas are specified. Recent work has identified evolutionary conserved enhancer elements that regulate gene expression in sharply demarcated regions in the ventricular zone of the E11.5 mouse cortex. The spatial extent of these regions is regulated by multiple transcription factors. Our lab is interested in identifying these transcription factors using mass spectroscopy. Our approach involves applying nuclear lysate from specific forebrain regions to enhancer elements. The proteins that specifically bind the enhancer will be isolated and identified using mass spectroscopy.